Microfluidic Diagnostics
Methods and Protocols
By: Gareth Jenkins (Editor), Colin D. Mansfield (Editor)
Hardcover | 18 January 2013
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Hardcover
540 Pages
540 Pages
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25.4 x 18.42 x 3.18
25.4 x 18.42 x 3.18
Hardcover
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Section 1: Microfluidic Diagnostics: From the Classroom to the Boardroom
1. Present Technology and Future Trends in Point of Care Microfluidic Diagnostics
Lawrence Kulinsky, Zahra Noroozi, and Marc Madou
2. Teaching Microfluidic Diagnostics Using Jell-O Chips
Cheng Wei T. Yang and Eric T. Lagally
3. Fundamentals of Microfluidics for High School Students with No Prior Knowledge of Fluid Mechanics
Vishal Tandon and Walter Peck
4. Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method
Chenren Shao and Don L. DeVoe
5. Overview of the Microfluidic Diagnostics Commercial Landscape
L. Kim
6. Practical Aspects of the Preparation and Filing of Patent Applications
Fiona Bessoth
7. Introduction to In Vitro Diagnostic Device Regulatory Requirements
Jonathan Day
Section 2. Microfluidic Diagnostics: Fabrication and Manipulation Protocols
8. Microfluidic Device Fabrication by Thermoplastic Hot-Embossing
Shuang Yang and Don L. DeVoe
9. Introduction to Glass Microstructuring Techniques
Radoslaw Mazurczyk, Colin Mansfield, Marcin Lygan
10.Glass Microstructure Capping and Bonding Techniques
Radoslaw Mazurczyk, Colin Mansfield, and Marcin Lygan
11. Rapid Prototyping of PDMS Device using SU-8 Lithography
Gareth Jenkins
12. Microfluidic Interface Technology Based on Stereolithography for Glass-based Lab-on-a-Chips
Song-I Han and Ki-Ho Han
13. Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays
Scott T. Phillips and Nicole K. Thom
14. Thread Based Devices for Low-Cost Diagnostics
Meital Reches
15. Droplet Based Microfluidics
Sanjiv Sharma, Monphichar Srisa-Art, Steven Scott, Amit Asthana, and Anthony Cass
16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET
Monipchar Srisa-Art and Sanjiv Sharma
17. Surface Treatments for Microfluidic Biocompatibility
N.J. Shirtcliffe and P. Roach
18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces
N.J. Shirtcliffe, and P. Roach
Section 3. Microfluidic Diagnostics: Application Protocols
19. The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries
Samantha M. Hattersley, John Greenman, and Stephen J. Haswell
20. Applications of Microfluidics for Molecular Diagnostics
Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale
21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethysiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers
Peng Li
22. Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry
Minseok S. Kim, Seyong Kwon, and Je-Kyun Park
23. Charged-couple Device (CCD) Detectors for Lab-on-a Chip (LOC) Optical Analysis
Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck
24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels
Michael P. Cuchiara and Jennifer L. West
25. Purifications of DNA/RNA in a Microfluidic Device
Andy Fan, Samantha Byrnes, and Catherine Klapperich
26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR
Xuefei Leng and Chaoyong James Yang
27. Intergrated Fluidic Circuits (IFCs) for Digital PCR
Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver
28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH)
Andrea Zanardi, Emanuele Barborini, and Roberta Carbone
29. An ELISA Lab-on-a-CHOP (ElISA-LOC)
Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov
30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis
Eric Ouellet, Louise Lund, and Eric T. Lagally
31. Surfa
Section 1. Microfluidic Diagnostics: From the Classroom to the Boardroom 1. Present Technology and Future Trends in Point of Care Microfluidic Diagnostics Lawrence Kulinsky, Zahra Noroozi, Marc Madou 2. Teaching Microfluidic Diagnostics Using Jell-O(R) Chips Cheng Wei T. Yang and Eric T. Lagally 3. Fundamentals of Microfluidics for High School Students with no Prior Knowledge of Fluid Mechanics Vishal Tandon and Walter Peck 4. Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method Chenren Shao and Don L. DeVoe 5. Overview of the Microfluidic Diagnostics Commercial Landscape L. Kim 6. Practical Aspects of the Preparation and Filing of Patent Applications Fiona Bessoth 7. Introduction to In Vitro Diagnostic Device Regulatory Requirements Jonathan Day Section 2. Microfluidic Diagnostics: Fabrication and Manipulation Protocols 8. Microfluidic Device Fabrication by Thermoplastic Hot-Embossing Shuang Yang and Don L. DeVoe 9. Introduction to Glass Microstructuring Techniques Radoslaw Mazurczyk and Colin Mansfield 10. Glass Microstructure Capping and Bonding Techniques Radoslaw Mazurczyk, Colin Mansfield, and Marcin Lygan 11. Rapid Prototyping of PDMS Devices using SUa'8 Lithography Gareth Jenkins 12. Microfluidic Interface Technology based on Stereolithography for Glass-based Lab-on-a-Chips Song-I Han and Ki-Ho Han 13. Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays Scott T. Phillips and Nicole K. Thom 14. Thread Based Devices for Low-Cost Diagnostics Meital Reches 15. Droplet Based Microfluidics Sanjiv Sharma, Monpichar Srisa-Art, Steven Scott , Amit Asthana, and Anthony Cass 16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET Monpichar Srisa-Art, Sanjiv Sharma 17. Surface Treatments for Microfluidic Biocompatibility N.J. Shirtcliffe, R. Toon, P. Roach 18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces N.J. Shirtcliffe, and P. Roach Section 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Samantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Minseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces N.J. Shirtcliffe, and P. Roach Section 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 17. Surface Treatments for Microfluidic Biocompatibility N.J. Shirtcliffe, R. Toon, P. Roach 18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces N.J. Shirtcliffe, and P. Roach Section 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Samantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Minseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Samantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Minseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET Monpichar Srisa-Art, Sanjiv Sharma 17. Surface Treatments for Microfluidic Biocompatibility N.J. Shirtcliffe, R. Toon, P. Roach 18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces N.J. Shirtcliffe, and P. Roach Section 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Samantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren Minseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces N.J. Shirtcliffe, and P. Roach Section 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces N.J. Shirtcliffe, and P. Roach Section 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell 20. Applications of Microfluidics for Molecular Diagnostics Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers Peng Li 22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 23. Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang 27. Integrated Fluidic Circuits (IFCs) for Digital PCR Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver 28. microFIND(R) Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone 29. An ELISA Lab-on-a-Chip (ELISA-LOC) Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov 30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis Eric Ouellet, Louise Lund, and Eric T. Lagally 31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement Kerstin Lange, Friederike J. Gruhl, and Michael Rapp 32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins Junjie Ou, Carolyn L. Ren 24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels Michael P. Cuchiara and Jennifer L. West 25. Purification of DNA/RNA in a Microfluidic Device Andy Fan, Samantha Byrnes, and Catherine Klapperich 26. Agarose Droplet Microfluidi
ISBN: 9781627031332
ISBN-10: 1627031332
Series: Methods in Molecular Biology
Published: 18th January 2013
Format: Hardcover
Language: English
Number of Pages: 540
Audience: Professional and Scholarly
Publisher: Springer Nature B.V.
Country of Publication: US
Dimensions (cm): 25.4 x 18.42 x 3.18
Weight (kg): 1.15
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