Microbial Steroids

Preface... Table of Contents... Contributing Authors... 1. Steroid Bioconversions Marina V. Donova 2. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins Ignacio Herraiz 3. Genome Sequencing of Steroid Producing Bacteria with Illumina Technology Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and marina V. Donova 4. Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome Alberto Sola-Landa, Antonio Rodriguez-Garcia, Carlos Barreiro, and Rosario Perez-Redondo 5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics Antonio Rodriguez-Garcia, Alberto Sola-Landa, and Carlos Barreiro 6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria Carlos Barreiro, Alejandro Morales, Ines Vazquez-Iglesias, and Alberto Sola-Landa 7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805 Jessica K. Loraine and Margaret C. M. Smith 8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas Jose M. Luengo and Elias R. Olivera 9. Selection of Biodegrading Phytosterol Strains Maria-Angelica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal 10. Stigmasterol Removal by an Aerobic Treatment System Soledad Chamorro, Claudia Xavier, Victor Hernandez, Jose Becerra, and Gladys Vidal 11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione Rodrigo A. Mancilla, Roberto Pavez-Diaz, and Andrea Munoz-Orrego 12. BETA-Sitosterol Bioconversion to Androstenedione in Microtiter Plates Marco P.C. Marques and Pedro Fernandez 13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium Kjell D. Josefsen, Anna Nordborg, and Havard Sletta 14. Scale-Up of Phytosterols Bioconversion into Androstenedione Sonia Martinez-Camara, Esther Bahillo, Jose-Luis Barredo, and Marta Rodriguez-Saiz 15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331 Julia Garcia-Fernandez, Igor Martinez, Lorena Fernandez-Cabezon, Carmen Felpeto-Santero, Jose-Luis Garcia, and Beatriz Galan 16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD) Surya Prakash and Abhay Bajaj 17. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett 18. Obtaining of 11alpha-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova 19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus Lidia Ortega-de los Rios, Jose M. Luengo, and Jose M. Fernandez-Canon 20. Biotransformation of DHEA into 7alpha, 15alpha-diOH-DHEA Hui Li, Jin Sun, and Zhenghong Xu 21. Stabilization of Enzymes by Using Thermophiles Ana-Luisa Ribeiro, Mercedes Sanchez, Aurelio Hidalgo, and Jose Berenguer 22. Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media Carla C. C. R. de Carvalho and Pedro Fernandes 23. Downstream Process Synthesis for Microbial Steroids Fabian B. Thygs and Juliane Merz 24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC) Govinda Guevara, Julian Perera, and Juana-Maria Navarro-Llorens 25. A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro Rabih Darwiche and Roger Schneiter