Methods in Molecular Biology

1. Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections Fernando Vazquez, Santido Melon, Luis Otero, and Maria de Ona 2. Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots Davide Gibellini, Elisa De Crignis, and Maria Carla Re 3. Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples Tony Bruns and Katrin Steinmetzer 4. Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis Linus Christerson and Bjorn Herrmann 5. Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens Sin Hang Lee 6. The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease Cheng-Yen Chen and Ronald C. Ballard 7. Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients R. B. Takkenberg, S. Menting, and M.G.H.M. Beld 8. Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance Mediating Mutations in Region V of the 23S rRNA Gene Jorgen Skov Jensen 9. Protocols for Detection and Typing of Treponema pallidum Using PCR Methods S.M. Bruisten 10. Protocol for the Extraction of DNA from Dried Blood in the Diagnosis of Convenital CMV Infection Jutte J.C. de Vries, Maria Barbi, Sandro Binda, and Eric C.J. Claas 11. Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis Beatrice Vitali, Elena Biagi, and Patrizia Brigidi 12. Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis Chung-Ter uhang and Shu-Ying Li 13. Protocol for the Detection and Genotyping of Human Papilloma viruses Using a Liquid Bead Microarray Assay (LBMA) Stephen Cherne and Qinghua Feng 14. Protocol for the Use of Enzyme-Linked Hybridisation Assays for Genital Ulcer Disease Seweryn Bialasiewicz, Ian M. Mackay, David M. Whiley, and Theo P. Sloots 15. Protocol for the Rapid Detection of the Urogential Tract Mollicutes and Chlamydia with Concomitant LGV -(sub)typing Nadine Mobius, Wibke Brenneisen, Anke Schaeffer, and Birgit Henrich 16. Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV Qian-Feng Xia 17. Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer Chou-Pong Pau, Susan K. Wells, and Timothy C. Granade 18. Protocol for the Use of Light Upon Extension (LUX) Real-Time PCR for the Determination of Viral Load in HBV Infection Guimin Li, Wangfeng Li, and Lixia Liu 19. Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations Sang-Myung Lee, Kyo Seon Hwang, Sang Kyung Kim, and Tae Song Kim 20. Protocol for the Detection of Treponema pallidum in Paraffin-Embedded SpecimensCheng-Yen Chen and Allan Pillay 21. Guidelines for the Use of Molecular Biological Methods to Detect Sexually Transmitted Pathogens in Cases of Suspected Sexual Abuse in Children Margaret R. Hammerschlag and Charlotte A. Gaydos 22. Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae Namraj Goire, Theo P. Sloots, Michael D. Nissen, and David M. Whiley 23. Protocol for a Facile Multiplex PCR for Multi-Antimicrobial Resistance and Gonococcus Detection Ratana Lawung, Rungrot Cherdtrakulkiat, and Virapong Prachayasittikul 24. Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae Lydgia A. Jackson and David W. Dyer 25. Protocol for the Detection of Human Leukocyte Antigen Variants and Sexually Transmitted Infections: From Genotyping to Immunoassays Jianming Tang and Anju Bansal 26. Protocol for the Clonal Analysis of NK Cell Effector Functions by Multi-Parameter Flow Cytometry Kathrin Schonberg, Maryam Hejazi, and Markus Uhrberg 27. Protocol for a Mammalian Cell-Based Assay for Monitoring the HIV-1 Protease Activity Chitra Rajakuberan, Brett J. Hilton, and Roland Wolkowicz 28. Self Collection of Specimens for Nucleic Acid-Based Diagnosis of Pharyngeal, Cevicovaginal, Urethral and Rectal Neisseria gonorrhoeae and Chlamydia trachomatis Infections Vivian Levy, Craig S. Blackmore, and Jeffrey D. Klausner 29. Sexually Transmitted Diseases - Reflections on Metaphors and Ethics Gisela Badura-Lotter