Methods in Molecular Biology

1. Introduction to Membrane Lipids Richard M. Epand 2. Introduction: Membrane properties (Good) for Life Marek Cebecauer 3. Introduction To Fluorescence Probing Of Biological Membranes Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mely 4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs) Charles Cranfield, Sonia Carne, Boris Martinac, and Bruce Cornell 5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells Deborah A. Brown 6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning KR Levental and I Levental 7. Asymmetric Giant Lipid Vesicle Fabrication Peichi C. Hu and Noah Malmstadt 8. Cholesterol Depletion using Methyl-b-cyclodextrin Saleemulla Mahammad and Ingela Parmryd 9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems G. Ekin Atilla-Gokcumen and Ulrike S. Eggert 10. Imaging Membrane Order using Environmentally Sensitive Fluorophores G Ashdown and DM Owen 11. 3D Super-resolution Imaging by Localisation Microscopy Astrid Magenau and Katharina Gaus 12. Electron Microscopy Methods for Studying Plasma Membranes Alison J. Beckett and Ian A. Prior 13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas 14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order Richard KP Benninger 15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes Eduard Hermann, Jonas Ries, and Ana J. Garcia-Saez 16. X-ray Diffraction of Lipid Model Membranes Arwen I.I. Tyler, Robert V. Law, and John M. Seddon 17. Solid State NMR of Lipid Model Membranes Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law 18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications Michael Carnell, Alex Macmillan, and Renee Whan 19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton 20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations Semen O. Yesylevskyy and Alexander P. Demchenko 21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers Svetlana Baoukina and D. Peter Tieleman