| Contributors | p. xi |
| Preface | p. xv |
| Volume in Series | p. xvii |
| Inflammation, Angiogenesis, and Lymphangiogenesis | p. 1 |
| Introduction | p. 2 |
| Angiogenesis and Lymphangiogenesis in Inflammation | p. 3 |
| Mouse Models of Inflammation | p. 5 |
| Commonly Used Markers of Blood Vessels and of Lymphatic Vessels | p. 8 |
| Measuring the (Lymph)angiogenic Response by Immunofluorescence | p. 10 |
| Quantitating (Lymph)angiogenesis by Fluorescence-Activated Cell Sorting | p. 12 |
| Measuring Vascular Permeability and Flow | p. 13 |
| Material and Methods | p. 14 |
| Acknowledgments | p. 20 |
| References | p. 20 |
| Color-Coded Fluorescent Mouse Models of Cancer Cell Interactions with Blood Vessels and Lymphatics | p. 27 |
| Introduction | p. 28 |
| Mouse Models: Fluorescent Tumors to Image Angiogenesis | p. 29 |
| Color-Coded Tumor-Host Models: GFP Nude Mouse | p. 32 |
| Color-Coded Tumor-Host Models: Nestin-Driven-GFP Nude Mouse | p. 36 |
| Imaging Cancer Cell Trafficking in Lymphatics: Experimental Metastasis Model | p. 37 |
| Imaging Cancer Cell Trafficking in Lymphatics: Spontaneous Metastasis Model | p. 39 |
| Protocol: Production of Fluorescent-Protein-Expressing Cancer Cell Lines | p. 41 |
| Protocol: Imaging of Cancer Cell Interactions with Blood Vessels and Lymphatics | p. 45 |
| Protocol: Imaging Methods for Mice Expressing Fluorescent Proteins | p. 47 |
| Summary and Conclusions | p. 49 |
| References | p. 50 |
| Bone Marrow-Derived Vascular Progenitors and Proangiogenic Monocytes in Tumors | p. 53 |
| Introduction | p. 54 |
| Methods for the Visualization of BMDCs in Tumors | p. 56 |
| Analysis of Pericyte Progenitors in Tumors | p. 66 |
| References | p. 79 |
| Screening Phage-Display Peptide Libraries for Vascular Targeted Peptides | p. 83 |
| Introduction | p. 84 |
| Methods | p. 90 |
| Concluding Remarks and Perspective | p. 100 |
| Acknowledgments | p. 101 |
| References | p. 101 |
| Avian Embryos: A Model for the Study of Primary Vascular Assembly in Warm-Blooded Animals | p. 107 |
| Introduction | p. 108 |
| Culture Insert Preparation | p. 109 |
| Culture Chamber Preparation | p. 109 |
| Construction of Microscope Incubator | p. 113 |
| Construction of Electroporation Chamber | p. 113 |
| Embryonic Culture Insert/Dish Preparation | p. 115 |
| Cell Labeling | p. 116 |
| Post-Incubation Fixation and Processing | p. 120 |
| Whole-Mount Immunolabeling | p. 121 |
| Plastic Embedding and Sectioning | p. 122 |
| References | p. 122 |
| Mouse Models to Investigate Anti-Cancer Effects of VEGF Inhibitors | p. 125 |
| Introduction | p. 125 |
| Xenografts | p. 127 |
| Genetic Models | p. 129 |
| Conclusions | p. 134 |
| References | p. 135 |
| Molecular Imaging of Tumor Vasculature | p. 141 |
| Introduction | p. 142 |
| Structural/Functional Imaging of Tumor Vasculature | p. 143 |
| Molecular Imaging | p. 148 |
| Imaging Integrin [alpha subscript v beta subscript 3] in Tumor Vasculature | p. 149 |
| Non-Radionuclide-Based Imaging of VEGFR in Tumor Vasculature | p. 153 |
| Radionuclide-Based Imaging of VEGFR in Tumor Vasculature | p. 156 |
| Experimental Section | p. 161 |
| Summary | p. 167 |
| Acknowledgments | p. 168 |
| References | p. 168 |
| Proteomic Mapping of the Vascular Endothelium In Vivo for Vascular Targeting | p. 177 |
| Introduction | p. 178 |
| Proteomics: An Overview | p. 180 |
| Proteomic Methodologies | p. 181 |
| Databases | p. 187 |
| Quantitative Proteomics | p. 188 |
| Application of Proteomics to the Vasculature | p. 192 |
| Summary | p. 198 |
| Protocols | p. 198 |
| References | p. 202 |
| Development of Coronary Vessels | p. 209 |
| Introduction | p. 210 |
| Microdissection and Explant Culture of the Proepicardium | p. 213 |
| Isolation of Total RNA from Individual PEs for Gene Expression Studies | p. 216 |
| Explant Culture of the Epicardium | p. 217 |
| Analysis of PE and Epicardium In Vivo by Scanning Electron Microscopy | p. 218 |
| Methods to Study Coronary Vessel Development In Vivo | p. 220 |
| Acknowledgments | p. 225 |
| References | p. 225 |
| Methods for Evaluating Uteroplacental Angiogenesis and Their Application Using Animal Models | p. 229 |
| Introduction | p. 230 |
| Importance of the Uteroplacental Circulation to Normal Fetal Growth and Development | p. 232 |
| Methods to Evaluate and Quantify Placental Angiogenesis | p. 235 |
| What we have Learned from Studies of Uteroplacental Angiogenesis Using Animal Models | p. 238 |
| Conclusions | p. 249 |
| Acknowledgments | p. 250 |
| References | p. 250 |
| Intravital Microscopic Investigation of Leukocyte Interactions with the Blood Vessel Wall | p. 255 |
| Introduction | p. 256 |
| Transillumination Intravital Microscopy | p. 256 |
| Measuring Leukocyte Rolling, Adhesion, and Transmigration | p. 259 |
| Spinning-Disk Confocal and Two-Photon Microscopy | p. 268 |
| Conclusion | p. 274 |
| References | p. 274 |
| Placental Remodeling of the Uterine Vasculature | p. 281 |
| Introduction | p. 282 |
| Isolation and Culture of Human Cytotrophoblasts | p. 285 |
| Isolation and Culture of First-Trimester, Human Placental Villous Explants | p. 290 |
| Identification of Cytotrophoblast-Modified Blood Vessels in Tissue Sections | p. 291 |
| Cytotrophoblast Migration and Induction of Endothelial Cell Apoptosis During Co-Culture | p. 293 |
| In Vitro Models of CTB Endovascular Invasion Using Explanted Spiral Arterioles | p. 295 |
| In Vivo Models of Human Cytotrophoblast Vascular Remodeling | p. 297 |
| References | p. 299 |
| An In Vivo Experimental Model for Postnatal Vasculogenesis | p. 303 |
| Introduction | p. 304 |
| Isolation of Blood-Derived Endothelial Progenitor Cells | p. 307 |
| Expansion and Characterization of Blood-derived Endothelial Progenitor Cells | p. 316 |
| Growth of Human Smooth Muscle Cells | p. 320 |
| In Vivo Vasculogenic Assay | p. 321 |
| Conclusion | p. 325 |
| Acknowledgments | p. 325 |
| References | p. 325 |
| Assessment of Arteriogenesis | p. 331 |
| Introduction | p. 332 |
| Anatomical Assessment of Arteriogenesis | p. 333 |
| Microangiography | p. 337 |
| Functional Assessment of Arteriogenesis | p. 339 |
| Laser-Doppler Perfusion Imaging | p. 339 |
| Electron Paramagnetic Resonance Tissue Oxymetry | p. 340 |
| Magnetic Resonance Imaging | p. 341 |
| Acknowledgments | p. 341 |
| References | p. 342 |
| Methods to Study Myeloid Cell Roles in Angiogenesis | p. 343 |
| Introduction | p. 344 |
| Methods for the Study of Myeloid Cells in Tumor Neovascularization and Growth | p. 347 |
| Conclusions | p. 369 |
| References | p. 369 |
| Author Index | p. 373 |
| Subject Index | p. 403 |
| Table of Contents provided by Ingram. All Rights Reserved. |
