Methods in Molecular Biology

Part 1. Identification and Typing of Legionella in the Environment and During Disease 1. Identification of Legionella in the EnvironmentNatalia A. Kozak, Claressa E. Lucas, and Jonas M. Winchell 2. Identification of Legionella in Clinical SamplesSophe Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, and Jerome Etienne 3. Sequence-Based Identification of LegionellaRodney M. Ratcliff 4. Investigation of Outbreaks - EpidemiologyCarol Joseph 5. Outbreak Investigations and Identification of Legionella in Contaminated WaterSusanne Lee and John Lee 6. Typing Methods for LegionellaChristian Luck, Norman K. Fry, Jurgen H. Helbig, Sophie Jarraud, and Timothy G. Harrison Part 2. Physiology and genetics of Legionella 7. Culture, Media and Handling of LegionellaChrista H. Chatfield and Nicholas P. Cianciotto 8. The Intracellular Metabolism of Legionella by Isotopologue ProfilingKlaus Heuner and Wolfgang Eisenreich 9. Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant ConstructionCarmen Buchrieser and Xavier Charpentier 10. Constructing Unmarked Gene Deletions in Legionella pneumophilaAndrew Bryan, Zachary D. Abbot, and Michele Swanson 11. Natural Biofilm Formation with Legionella pneumophilaEmilie Portier and Yann Hechard 12. Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration MediumSophie Pecastaings and Christine Roques 13. Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophilaJens Jager and Michael Steinert Part 3. Cellular microbiology of Legionella 14. Analysis of Legionella Infection by Flow CytometryAndre Tiaden, Aline Kessler, and Hubert Hilbi 15. Analysis of Legionella Infection Using RNAi in Drosophila CellsDennise A. De Jesus, Tamara J. O'Connor, and Ralph R. Isberg 16. Immunofluorescence Imaging of Legionella Infection In vitroStefan S. Weber and Annette Oxenius 17. Electron Microscopy of Legionella and Legionella-Infected CellsGary Faulkner and Raphael Garduno 18. Pathogen Vacuole Purification from Legionella-Infected Amoeba and MacrophagesChristine Hoffmann, Ivo Finsel, and Hubert Hilbi 19. Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion SystemWenhan Zhu and Zhao-Qing Luo 20. Subcellular Localization of Legionella Dot/Icm EffectorsAdam J. Vogrin, Aurelie Mousnier, Gad Frankel, and Elizabeth L. Hartland Part 4. Biochemical assays to study Legionella effectors and enzymes 21. Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligaseHiroki Nagai and Tomoko Kubori 22. Legionella Phospholipases Implicated in Infection - Determination of Enzymatic ActivitiesKathleen Seipel and Antje Flieger 23. Interactions of Legionella Effector Proteins with Host Phosphoinositide LipidsStephen Weber, Stephanie Dolinsky, and Hubert Hilbi 24. Characterization of Legionella lipopolysaccharideChristian Luck and Jurgen Helbig Part 5. Immunity and host response against Legionella 25. The Tetrahymena and Acanthamoeba Model SystemsSharon G. Berk and Rafael A. Garduno 26. Dictyostelium Host Response to Legionella infection: Strategies and AssaysSalvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger 27. The Caenorhabditis elegans Model of Legionella InfectionAnn Karen C. Brassinga and Costi D. Sifri 28. Infection of Nonphagocytic Host Cells by LegionellaShandra R. Day, Costi D. Sifri, and Paul S. Hoffman 29. Analyzing Caspase-1 Activation During Legionella pneumophila Infection in MacrophagesChristopher L. Case and Craig R. Roy 30. The Mouse as a Model for Pulmonary Legionella InfectionJuliana I. Hori and Dario Zamboni 31. Assessment of Legionella-Specific Immunity in MiceStefan Weber, Nicole Joller, and Annette Oxenius 32. The Guinea Pig Model of Legionnaires' DiseasePaul H. Edelstein 33. Human Susceptibility to Legionnaires' DiseaseWilliam R. Berrington and Thomas R. Hawn Part 6. Genomics, transcriptomics and proteomics of Legionella 34. cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophilaTobias Sahr and Carmen Buchrieser 35. Methods to Study Legionella Transcriptome in vitro and in vivoSebastian Faucher and Howard Shuman 36. Co-Immunoprecipitation: Protein-RNA and Protein-DNA InteractionTobias Sahr and Carmen Buchrieser 37. Identification of Legionella Effectors Using Bioinformatic ApproachesGil Segal Part 5. Immunity and host response against Legionella 25. The Tetrahymena and Acanthamoeba Model SystemsSharon G. Berk and Rafael A. Garduno 26. Dictyostelium Host Response to Legionella infection: Strategies and AssaysSalvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger 27. The Caenorhabditis elegans Model of Legionella InfectionAnn Karen C. Brassinga and Costi D. Sifri 28. Infection of Nonphagocytic Host Cells by LegionellaShandra R. Day, Costi D. Sifri, and Paul S. Hoffman 29. Analyzing Caspase-1 Activation During Legionella pneumophila Infection in MacrophagesChristopher L. Case and Craig R. Roy 30. The Mouse as a Model for Pulmonary Legionella InfectionJuliana I. Hori and Dario Zamboni 31. Assessment of Legionella-Specific Immunity in MiceStefan Weber, Nicole Joller, and Annette Oxenius 32. The Guinea Pig Model of Legionnaires' DiseasePaul H. Edelstein 33. Human Susceptibility to Legionnaires' DiseaseWilliam R. Berrington and Thomas R. Hawn Part 6. Genomics, transcriptomics and proteomics of Legionella 34. cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophilaTobias Sahr and Carmen Buchrieser 35. Methods to Study Legionella Transcriptome in vitro and in vivoSebastian Faucher and Howard Shuman 36. Co-Immunoprecipitation: Protein-RNA and Protein-DNA InteractionTobias Sahr and Carmen Buchrieser 37. Identification of Legionella Effectors Using Bioinformatic ApproachesGil Segal