Lab Ref: v. 2

Introduction SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSIS Isolation of Total DNA Isolation of Total RNA Selection of Poly(A) RNA from Total Isolation and Amplification of RNA from Single Cells and Small Amounts of Tissue Hybridization Analysis Preparation of Nucleic Acid (Target) for Array Hybridization Analysis Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis Other Quantitative Methods SECTION 2: RNAi RNAi in C. elegans RNAi in D. melanogaster RNAi in Plants RNAi in Mammalians RNAi in Mouse Oocytes and Early Embryos SECTION 3: IMAGING Preparation of Embryos for Live Cell Imaging Imaging Fixed Tissue Preparation of Mice for Imaging Intracellular Imaging General Information for Imaging SECTION 4: PROTEIN ANALYSIS/PROTEOMICS Basic Methods in Protein Analysis for Proteomics Extraction of Proteins Advanced Protocols in Identifying Protein-Protein Interactions for Proteomics SECTION 5: ARABIDOPSIS Microscopy Genetic Manipulation Protein Extraction Nuclear Extraction Histochemistry of GUS Fusion SECTION 6: MURINE In Vitro Culturing of Embryonic Stem Cells Genotyping Immunohistochemistry of Whole-mount Embryos Other Buffers and Solutions SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLES SECTION 8: USEFUL FACTS AND TABLES A. Useful Information and Reference Tables B. Nomenclature Guide C. Useful World Wide Web Sites Appendix Index