Immunochemical Protocols

Immunization Strategies for Antibody Production Robert Burns Synthetic Peptides as Antigens for Antibody Production David C. Hancock and Nicola J. O'Reilly Production of Polyclonal Antibodies in Rabbits David C. Hancock and Nicola J. O'Reilly Making Hybridomas Robert Burns Growing Hybridomas Gary Entrican and Gareth Young Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments Michael Hust and Stefan D¼bel Purification of Antibodies and Preparation of Antibody Fragments Graham D. Kemp Conjugation of Antibodies to Alkaline Phosphatase G. Brian Wisdom Conjugation of Antibodies to Horseradish Peroxidase G. Brian Wisdom Conjugation of Antibodies to Fluorescein or Rhodamine G. Brian Wisdom Conjugation of Antibodies to Biotin James D. Hirsch and Rosaria P. Haugland Preparation of Colloidal Gold Probes David Hughes Immunogold Probes in Light Microscopy David Hughes Immunoelectron Microscope Techniques in Plant Virus Diagnosis Daphne M. Wright Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay William Jordan Competitive Enzyme-Linked Immunosorbent Assay William Jordan Immunoblotting Techniques Barbara Magi and Sabrina Liberatori Epitope Mapping Glenn E. Morris Generation and Testing of Immunoaffinity Columns Weilin L. Shelver Immunocapture-Polymerase Chain Reaction Vincent Mulholland Magnetic Cell Sorting Maciej Zborowski and Jeffrey J. Chalmers Cell Enrichment and Immunochemical Staining Oscar R. Lara and Jeffrey J. Chalmers Index