Host-Pathogen Interactions

1. Genetic Association Studies in Host-Pathogens Interaction Analysis Jose Luis Royo and Luis Miguel Real 2. Bacterial Genotyping Methods: From the Basics to Modern Margarita M. Ochoa-Diaz, Silvana Daza-Giovannetty, and Doris Gomez-Camargo Part I: Animal-Pathogen Interaction Section 3. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens Gili Aviv and Ohad Gal-Mor 4. The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection Gili Aviv and Ohad Gal-Mor 5. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens Gili Aviv and Ohad Gal-Mor 6. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity Gili Aviv and Ohad Gal-Mor 7. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage Carlos Medina, Beatriz Mesa-Pereira, Eva M Camacho, Amando Flores, and Eduardo Santero 8. Organoids as a Model to Study Infectious Disease Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, and Eitaro Aihara 9. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins Anita Horvatic, Josipa Kules, Nicolas Guillemin, Franjo Martinkovic, Iva Stimac, Vladimir Mrljak, and Mangesh Bhide 10. Systems Biology Modeling to Study Pathogen-Host Interactions Muberra Fatma Cesur and Saliha Durmus 11. Phage Therapy: Various Perspectives on How to Improve the Art Stephen T. Abedon Part II: Microbe-Microbe Interaction Section 12. Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression Cristina Isabel Amador, Claus Sternberg, and Lars Jelsbak 13. Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth Fernando Govantes 14. Detection of Bacterial Quorum Sensing Molecules Elke Stein and Adam Schikora Part III: Plant-Pathogen Interaction Section 15. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae Jose S. Rufian, Diego Lopez-Marquez, Nieves Lopez-Pagan, Murray Grant, Javier Ruiz-Albert, and Carmen R. Beuzon 16. Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation Anthony Perrier, Patrick Barberis, and Stephane Genin 17. In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors Fabien Lonjon, Nemo Peeters, Stephane Genin, and Fabienne Vailleau 18. Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains Alice Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthome, and Alice Guidot 19. Methods to Quantify Biotic-Induced Stress in Plants Marcel Bach-Pages and Gail M. Preston 20. From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy Helen N. Fones and George R. Littlejohn 21. Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria Jochen Schmid, Broder Ruhmann, Volker Sieber, Lorena Romero-Jimenez, Juan Sanjuan, and Daniel Perez-Mendoza 22. Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria Marta Robledo, Natalia I. Garcia-Tomsig, and Jose I. Jimenez-Zurdo 23. A New, Non-Destructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean Angeles Hidalgo, Jose E. Ruiz-Sainz, and Jose M. Vinardell 24. Methods for the Characterization of Plant-Growth Promoting Rhizobacteria Md. Motaher Hossain and Farjana Sultana