Methods in Molecular Biology

By: D. Lansing Taylor (Editor)

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Hardcover | 15 August 2006

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There has always been some tension between proponents of hypothesis-driven and discovery-driven research in the broad field of life sciences. Academic research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery research in the pharmaceutical industry has embraced discovery-driven research for many years. A good example has been the discovery of active compounds from large chemical libraries, through screening campaigns. The success of the human genome project has also demonstrated the need for both academic researchers and industrial researchers to now understand the functions of genes and gene products. The cell is the basic unit of life and it has been at the cellular level where function can be demonstrated most cost-effectively and rapidly. High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell.

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Non-Fiction Computing & I.T.Computer Science Image Processing Medicine Other Branches of Medicine Pharmacology Nursing & Ancillary Services Pharmacy & Dispensing Science Biology, Life Sciences Cellular Biology

Molecular Biology Pre-Clinical Medicine & Basic Sciences Medical Genetics Biochemistry

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Published: 10th December 2010

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Preface	p. v
Contributors	p. xi
Guide to the Companion CD	p. xiii
Introduction to High Content Screening
Past, Present, and Future of High Content Screening and the Field of Cellomics	p. 3
A Pharmaceutical Company User's Perspective on the Potential of High Content Screening in Drug Discovery	p. 19
Linking Microscopy and High Content Screening in Large-Scale Biomedical Research	p. 33
Instrumentation, Biological Application Software, and Sample Preparation
Requirements, Features, and Performance of High Content Screening Platforms	p. 41
Characteristics and Value of Directed Algorithms in High Content Screening	p. 63
Characteristics and Value of Machine Learning for Imaging in High Content Screening	p. 83
Tools for Quantitative and Validated Measurements of Cells	p. 95
Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays	p. 109
Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications	p. 121
Use of the CellCard(tm) System for Analyzing Multiple Cell Types in Parallel	p. 129
Reagents
Reagents to Measure and Manipulate Cell Functions	p. 141
Fluorescent Proteins and Engineered Cell Lines	p. 165
Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays	p. 189
The HaloTag(tm): A Novel Technology for Cell Imaging and Protein Analysis	p. 195
Protein Labeling With FlAsH and ReAsH	p. 209
Exploiting Network Biology to Improve Drug Discovery	p. 221
Physiological Indicators of Cell Function	p. 233
The Use of siRNA to Validate Immunofluorescence Studies	p. 245
Caged Substrates Applied to High Content Screening: An Introduction With an Eye to the Future	p. 253
Informatics and Bioinformatics
Overview of Informatics for High Content Screening	p. 269
Large-Scale Data Management for High Content Screening	p. 281
An Integrated Biomedical Knowledge Extraction and Analysis Platform: Using Federated Search and Document Clustering Technology	p. 293
Visualization of High Content Screening Data	p. 301
Pathway Mapping Tools for Analysis of High Content Data	p. 319
Assays and Applications of High Content Screening
Systems Biology in Cancer Research: Genomics to Cellomics	p. 353
Target Validation in Drug Discovery	p. 367
High Content Screening as a Screening Tool in Drug Discovery	p. 379
Discovery of Protein Kinase Phosphatase Inhibitors	p. 389
High Content Translocation Assays for Pathway Profiling	p. 401
In Vitro Cytotoxicity Assessment	p. 415
Neurite Outgrowth in Retinal Ganglion Cell Culture	p. 427
Index	p. 435
Table of Contents provided by Publisher. All Rights Reserved.

Methods in Molecular Biology

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