Part I. Overview 1. The Hematopoietic Stem Cell Landscape Kevin D. Bunting and Cheng-Kui Qu Part II. Stem Cell Enrichment and Heterogeneity 2. Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow Huimin Cao, Brenda Williams, and Susan K. Nilsson 3. Single-Cell PCR Profiling of Gene Expression in Hematopoiesis Jose Teles, Tariq Enver, and Cristina Pina 4. Hematopoietic Stem and Progenitor Cell Mobilization in Mice Jonathan Hoggatt, Tiffany A. Tate, and Louis M. Pelus 5. Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells Brahmananda Reddy Chitteti and Edward F. Srour 6. Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells Lei Dong and Cheng-Kui Qu Part III. Molecular and Cellular Characterization 7. Gene Expression Profiling of Hematopoietic Stem Cells (HSC) Nalini Raghavachari 8. Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells Wenhuo Hu and Christopher Y. Park 9. DNA Methylation Profiling of Hematopoietic Stem Cells Amber H . Begtrup 10. Metabolic Characterization of Hematopoietic Stem Cells Fatih Kocabas, Junke Zheng, Chengcheng Zhang, and Hesham A. Sadek 11. NanoProteomic Assays on Hematopoietic Stem Cells Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, and Kevin D. Bunting Part IV. In vitro Assays and Differentiation 12. Hematopoietic Differentiation of Pluripotent Stem Cells in Culture Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, and Deborah L. French 13. Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, and Jan Jacob Schuringa 14. Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells Phuong L. Doan and John P. Chute 15. Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level Byungwook Aha, Zhengqi Wang, David R. Archer, and Wilbur A. Lam Part V. Transplantation Assays and Imaging Engraftment 16. Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells Ryo Yamamoto, Yohei Morita, and Hiromitsu Nakauchi 17. Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull Juwell W. Wu, Judith M. Runnels, and Charles P. Lin 18. Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development Ken-Edwin Aryee, Leonard D. Shultz, and Michael A. Brehm 19. Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, and Linheng Li Part VI. Genetic Modification 20. Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, and David A. Williams 21. Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells Niek P. van Til and Gerard Wagemaker 22. High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, and Hans-Peter Kiem 23. Barcoded Vector Libraries and Retro- or Lenti-Viral Barcoding of Hematopoietic Stem Cells Leonid V. Bystrykh, Gerald de Haan, and Evgenia Verovskaya
