Germline Development

Part I: Male Germline 1. Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studies Yunmin Li, Teruko Taketo, and Yun-Fai Chris Lau 2. Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis Gurpreet Manku, Monty Mazer, and Martine Culty 3. Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter Mice Thomas Garcia and Marie-Claude Hofmann 4. Isolation of Human Male Germline Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell Sorting Zuping He, Maria Kokkinaki, Jiji Jiang, Wenxian Zeng, Ina Dobrinski, and Martin Dym 5. Isolation and Purification of Murine Male Germ Cells Catherine Boucheron and Vanessa Baxendale 6. Preparation of Enriched Mouse Syncitia-Free Pachytene Spermatocyte Cell Suspensions Yan Wang and Martine Culty 7. Revealing Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan 8. Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase Alan Lap-Yin Pang 9. Identification of Novel Long Non-Coding RNA Transcripts in Male Germ Cells Tin-Lap Lee, Amy Xiao, and Owen M. Rennert 10. Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization Hoi-Hung Cheung, Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan 11. Spermatogenesis in Cryptorchidism Alexander I. Agoulnik, Zaohua Huang, and Lydia Ferguson Part II: Female Germline 12. In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development Shyamal K. Roy, Cheng Wang, Anindit Mukherjee, and Prabuddha Chakraborty 13. Microspread Oocyte Preparations for the Analysis of Meiotic Prophase Progression with Improved Recovery by Cytospin Centrifugation Teruko Taketo 14. In Vitro Maturation (IVM) of Porcine Oocytes Ye Yuan and Rebecca L. Krisher 15. Experimental Approaches to the Study of Human Primordial Germ Cells Andrew J. Childs and Richard A. Anderson 16. Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and Immunohistochemistry Chang Liu, Melissa Paczkowski, Manal Othman, and Humphrey Hung-Chang Yao 17. DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods Hisato Kobayashi and Tomohiro Kono 18. Gene Expression in Mouse Oocytes by RNA-Seq Eric Antoniou and Robert Taft