Gene Synthesis

Section I: Assembly of Oligonucleotides in Synthons 1. Building Block Synthesis using the Polymerase Chain Assembly (PCA) Method Julie A. Marchand and Jean Peccoud 2. Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments Daniel G. Gibson 3. TopDown Real-Time Gene Synthesis Mo Chao Huang, Wai Chye Cheong, Hongye Ye, Mo-Huang Li 4. De novo DNA Synthesis using Single-molecule PCR Tuval Ben Yehezkel, Gregory Linshiz, and Ehud Shapiro Section II: Synthon Assembly 5. SLIC: A Method for Sequence and Ligation Independent Cloning Mamie Z. Li and Stephen J. Elledge 6. Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli Olivia Ho-Shing, Kin Lau, William Vernon, Todd T. Eckdahl, and A. Malcolm Campbell 7. Assembling DNA Fragments by USER Fusion Narayana Annaluru, Heloise Muller, Sivaprakash Ramalingam, Karthikeyan Kandavelou, Viktoriya London, Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper, Joel S. Bader, Jef D. Boeke and Srinivasan Chandrasegaran 8. Fusion PCR via Novel Overlap Sequences Kamonchai Cha-aim, Hisashi Hoshida, Tomoaki Fukunaga, and Rinji Akada 9. Using Recombineering to Generate Point Mutations: the Oligonucleotide-based "Hit & Fix" Method Suhwan Chang, Stacey Stauffer and Shyam K. Sharan 10. Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method Kajal Biswas, Stacey Stauffer and Shyam K. Sharan 11. Assembling Large DNA Segments in Yeast Heloise Muller, Narayana Annaluru, Joy Wu Schwerzmann ,Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper, Joel S. Bader, Jef D. Boeke, Srinivasan Chandrasegaran 12. Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides Gregory Linshiz Tuval Ben Yehezkel and Ehud Shapiro 13. Cloning Whole Bacterial Genomes in Yeast Gwynedd A. Benders 14. Production of Infectious Poliovirus from Synthetic Viral Genomes Jeronimo Cello and Steffen Mueller Section III: Software for gene synthesis 15. In Silico Design of Functional DNA Constructs Alan Villalobos, Mark Welch, and Jeremy Minshull 16. Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis David Hoover 17. De Novo Gene Synthesis Design Using TmPrime Software Mo-Huang Li, Marcus Bode, Mo Chao Huang, Wai Chye Cheong, Li Shi Lim 18. Design-A-Gene with GeneDesign Sarah M. Richardson, Steffi Liu, Jef D. Boeke, and Joel S. Bader Section IV: Education and Security 19. Leading a Successful iGEM Team Wayne Materi 20. The Build-a-Genome Course Eric M. Cooper, Heloise Muller, Srinivasan Chandrasegaran, Joel Bader, and Jef D. Boeke 21. DNA Synthesis Security Ali Nouri and Christopher F. Chyba