Fed-Batch Fermentation

A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli

By: G G Moulton

Write A Review

Hardcover | 30 September 2014 | Edition Number 1

At a Glance

Hardcover
188 Pages

Dimensions(cm)
24.13 x 15.88 x 1.91

Hardcover

RRP $350.95

$311.75

11%OFF

or 4 interest-free payments of $77.94 with

or

Ships in 5 to 7 business days

Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

This process can be used as a teaching tool for the inexperienced fermentation student or researcher in the fields of bioprocessing and bioreactors. It is an important segue from E. coli shake flask cultures to bioreactor
The fed-batch fermentation is designed to be accomplished in a single day with the preparation work being done on the day prior
The fed-batch fermentation described in this book is a robust process and can be easily scaled for CMO production of protein product

Shipping

	Standard Shipping	Express Shipping
Metro postcodes:	$9.99	$14.95
Regional postcodes:	$9.99	$14.95
Rural postcodes:	$9.99	$14.95

Orders over $79.00 qualify for free shipping.

How to return your order

At Booktopia, we offer hassle-free returns in accordance with our returns policy. If you wish to return an item, please get in touch with Booktopia Customer Care.

Additional postage charges may be applicable.