Get Free Shipping on orders over $79
Expression Systems : Methods Express - Michael Dyson

Expression Systems

Methods Express

By: Michael Dyson (Editor), Yves Durocher (Editor)

Hardcover | 15 October 2007 | Edition Number 1

At a Glance

Hardcover


RRP $185.00

$176.75

or 4 interest-free payments of $44.19 with

 or 

Available for Backorder. We will order this from our supplier however there isn't a current ETA.

Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors
*CHO cells
* E. coli
*HEK293-EBNA1 cells
* Lactococcus lactis and other gram positive bacteria
* S. cerevisiae
*transfected insect cells

* Pichia pastoris
*mammalian cells using BacMam viruses
*lentiviral vectors
*wheat germ cell-free system

The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.

Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.

In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.

More in Life Sciences in General

Liars, cheats and copycats : Trickery and deception in nature - James O'Hanlon
Is a River Alive? - Robert Macfarlane

RRP $26.99

$22.99

15%
OFF
A World Appears : A Journey Into Consciousness - Michael Pollan

RRP $39.99

$31.75

21%
OFF
Gray's Anatomy for Students : 5th Edition - Richard Drake

RRP $159.95

$121.75

24%
OFF
Behave : The Biology of Humans at Our Best and Worst - Robert M Sapolsky
The Dawn of Everything : A New History of Humanity - David Graeber
Dopamine Nation : Why our Addiction to Pleasure is Causing us Pain - Anna Lembke
Environmental Biology : 1st Edition - Mike Calver

RRP $184.95

$144.75

22%
OFF
How To Change Your Mind : New Science of Psychedelics - Michael Pollan
Swainston's Fishes of Australia : The complete illustrated guide - Roger Swainston
Sapiens : A Graphic History: Volume 1 - Yuval Noah Harari

RRP $39.99

$31.75

21%
OFF
Anatomy & Physiology For Dummies : For Dummies - Erin Odya

RRP $38.95

$27.27

30%
OFF
Sapiens A Graphic History, Volume 2 : The Pillars of Civilization - Yuval Noah Harari