Embryonic Stem Cell Protocols

1. Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition Jennifer Nichols and Thorsten Boroviak 2. A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Keitaro Imaizumi, Momoe Iha, Naoki Nishishita, Shin Kawamata, Shinichi Nishikawa, and Teruo Akuta 3. Resolving Heterogeneity: Fluorescence Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture Jurriaan J. Holzenspies, Gelo V. Dela Cruz, and Joshua M. Brickman 4. Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium Matteo Tosolini and Alice Jouneau 5. Generation of Embryonic Stem Cells in Rabbits Marielle Afanassieff, Pierre Osteil, and Pierre Savatier 6. Gene Transfer into Pluripotent Stem Cells Via Lentiviral Transduction Ortwin Naujok, Ulf Diekmann, and Matthias Elsner 7. Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells Anna Pezzarossa, Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches 8. Expanding the Utility of FUCCI Reporters Using FACS-Based 'Omics Analysis James Chappell, Ben Boward, and Stephen Dalton 9. Selection of Antibodies Interfering with Cell-Surface Receptor Signalling Using Embryonic Stem Cell Differentiation Anna N. Melidoni, Michael R. Dyson, and John McCafferty 10. Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells Lida Radan, Chris S. Hughes, Jonathan H. Teichroeb, Lynne-Marie Postovit, and Dean H. Betts 11. Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells Anisa B. Rahim and Leah A. Vardy 12. Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells Ulf Diekmann and Ortwin Naujok 13. Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume 14. Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers Atsumasa Okada, Katsuhisa Tashiro, Tomoko Yamaguchi, and Kenji Kawabata 15. Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells John Avery and Stephen Dalton 16. From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells Matteo Tosolini and Alice Jouneau 17. In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production Iliana Fauzi, Nicki Panoskaltsis, and Athanasios Mantalaris 18. Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes M. Oktar Guloglu and Anna Larsen 19. Intracellular Calcium Measurements for Functional Characterization of Neuronal Phenotypes Talita Glaser, Ana Regina G. Castillo, Agatha Oliveira, and Henning Ulrich 20. In Vitro Differentiation of Pluripotent Stem Cells into Functional ss Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets Bipasha Bose and Sudheer Shenoy P 21. Generation of Corneal Keratocytes from Human Embryonic Stem Cells Andrew J. Hertsenberg and James L. Funderburgh 22. Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells Tetsuya S. Tanaka 23. Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool Rahel Wettstein, Maxime Bodak, and Constance Ciaudo 24. Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis Ivan Roubal, Sun Joo Park, and Yong Kim 25. 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells Malin Hernebring 26. A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells Peter F. Renz and Tobias A. Beyer 27. Applying Shear Stress to Pluripotent Stem Cells Russell P. Wolfe, Julia B. Guidry, Stephanie L. Messina, and Tabassum Ahsan 28. Generating Inner Ear Organoids from Mouse Embryonic Stem Cells Emma Longworth-Mills, Karl R. Koehler, and Eri Hashino 29. Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells Ixchelt Cuaranta-Monroy, Zoltan Simandi, and Laszlo Nagy 30. Pancreatic Differentiation from Murine Embryonic Stem Cells Daisuke Sakano, Nobuaki Shiraki, and Shoen Kume 31. Osteogenic Differentiation from Embryonic Stem Cells Yanhong Yu, Carlos Pilquil, and Michal Opas 32. Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells Kishore Reddy Katikireddy and Ula V. Jurkunas