| Preface | p. v |
| Contributors | p. xi |
| Cytochrome P450 Nomenclature, 2004 | p. 1 |
| Spectral Analyses of Cytochromes P450 | p. 11 |
| Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli | p. 19 |
| Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems | p. 31 |
| Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System | p. 39 |
| Cytochrome P450 Reconstitution Systems | p. 61 |
| Catalytic Assays for Human Cytochrome P450: An Introduction | p. 73 |
| Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate | p. 85 |
| Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation | p. 91 |
| Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes | p. 97 |
| High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6[alpha]-Hydroxylation | p. 103 |
| Determination of CYP2C9-Catalyzed Diclofenac 4'-Hydroxylation by High-Performance Liquid Chromatography | p. 109 |
| CYP2C19-Mediated (S)-Mephenytoin 4'-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection | p. 115 |
| CYP2D6-Dependent Bufuralol 1'-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection | p. 121 |
| Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation | p. 127 |
| Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6[beta]-Hydroxylation | p. 133 |
| Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection | p. 143 |
| An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7[alpha]-Hydroxylation | p. 149 |
| Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families | p. 153 |
| Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity | p. 157 |
| Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases | p. 163 |
| Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes | p. 173 |
| The Human Cytochrome P450 Allele Nomenclature Committee Web Site: Submission Criteria, Procedures, and Objectives | p. 183 |
| Genotyping for Cytochrome P450 Polymorphisms | p. 193 |
| Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing | p. 209 |
| Isolation of Rat Hepatocytes | p. 229 |
| Rat Hepatocyte Cultures: Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells | p. 239 |
| Rat Hepatocyte Cultures: Collagen Gel Sandwich and Immobilization Cultures | p. 247 |
| Hepatocytes in Suspension | p. 255 |
| Isolation of Rat Bone Marrow Stem Cells | p. 265 |
| Transfection of Primary Cultures of Rat Hepatocytes | p. 273 |
| Human Hepatocyte Culture | p. 283 |
| Determination of Cellular Localization of the Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization | p. 295 |
| Deletion of Genes From the Mouse Genome Using Cre/loxP Technology | p. 307 |
| Characterization of Targeted Mouse Embryonic Stem Cell Chromosomes: Karyotyping and Fluorescence In Situ Hybridization of Metaphase Spreads | p. 321 |
| Microinjection of Targeted Embryonic Stem Cells and Establishment of Knockout Mouse Lines for Fmo Genes | p. 329 |
| Use of Reporter Genes to Measure Xenobiotic-Mediated Activation of CYP Gene Transcription | p. 343 |
| Index | p. 355 |
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