Current Protocols Essential Laboratory Techniques

Preface 

Contributors List

Conversion Factors

Flow Chart: Application of Techniques
Emily Wiley, Claremont Colleges

Chapter 1 Volume/Weight Measurement

Unit 1.1 Volume Measurement
Andrew Zanella and Tom Davis, Claremont Colleges

Unit 1.2 Weight Measurement
Michael Guzy, Ohaus, Corp.

Chapter 2 Concentration Measurement

Unit 2.1 Spectrophotometry
Rob Morris, Ocean Optics, Inc.

Unit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Sean R. Gallagher, UVP, LLC

Chapter 3 Reagent Preparation

Unit 3.1 Theoretical Discussion
Deb Chakravarti, Keck Graduate Institute

Unit 3.2 Practical Discussion
Deb Chakravarti, Keck Graduate Institute

Unit 3.3 Recipes

In-House

Chapter 4 Cell Culture Techniques

Unit 4.1 Aseptic Technique
Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine

Unit 4.2 Culture of Escherichia coli and Related Bacteria
Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine

Chapter 5 Sample Preparation

Unit 5.1 Centrifugation and Cell Fractionation In-House

Unit 5.2 Purification and Concentration of Nucleic Acids
Dennis H. Dowhan, University of Queensland

Chapter 6 Chromatography

Unit 6.1 Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences

Unit 6.2 Column Chromatography
Deb Chakravarti, Keck Graduate Institute

Chapter 7 Electrophoresis

Unit 7.1 Overview of Electrophoresis
Sean R. Gallagherr, UVP, LLC

Unit 7.2 Agarose Gel Electrophoresis
Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College

Unit 7.3 SDS-PAGE
Sean Gallagher, UVP, LLC

Unit 7.4 Staining of Gels
Sean Gallagher, UVP, LLC

Chapter 8 Blotting

Unit 8.1 Overview of Blotting
Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute

Unit 8.2 Nucleic Acid Blotting: Southern and Northern
Laura L. Mays Hoopes, Pomona College

Unit 8.3 Protein Blotting: Immunoblotting
Sean Gallagher, UVP, LLC

Unit 8.4 Labeling DNA and Probe Preparation
Karl A. Haushalter, Harvey Mudd College

Chapter 9 Microscopy

Unit 9.1 Conventional Light Microscopy
Eric S. Cole, St. Olaf College

Unit 9.2 Immunofluorescence Microscopy
David J. Asai, Harvey Mudd College

Chapter 10 Enzymatic Reactions

Unit 10.1 Working with Enzymes
David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp.

Unit 10.2 Overview of PCR
Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute

Unit 10.3 Real-Time PCR
Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University

Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast
Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College