Editor
List of contributors
Foreword
Preface
SECTION I Cryo-EM in Cell Biology: Structure Determination of Protein Assemblies and Cellular Organelle
Chapter 1 Introduction on Cryogenic Electron Microscopy in Structural Biology
Krishnarao Appasani
Chapter 2 Structural Dynamics of Proteasome Regulation by Cryo-EM
Youdong Mao
Chapter 3 Cryo-EM Structures of a Ubiquitin-Specific Protease in Complex with Substrate
Martin Rennie
Chapter 4 Structural Biology of Protein Kinases and Their Regulation
Samrat Mitra, Pranita Ray, Prateeka Borar, and Smarajit Polley
Chapter 5 The Life and Times of p53: Structural Advancements to Decipher the Guardian of the Genome
Maria J. Solares, Samantha Berry, and Deborah F. Kelly
SECTION II Technology Platform and Evolving Technologies
Chapter 6 Single-Particle Cryo-EM: What Happens Inside the Black Box?
Sayan Bhakta and Marin van Heel
Chapter 7 Streptavidin Affinity Grids in the Study of Macromolecular Complexes Using Cryo-Electron Microscopy
Nils Marechal and Patrick Schultz
Chapter 8 Running a Cost-Effective Modern Cryo-EM Facility for SPA Analysis
Lijia Jia
Chapter 9 Computational Methods for Bimolecular Structure Modeling for Cryo-EM
Javad Baghirov, Xiao Wang, Genki Terashi, Shu Li, and Daisuke Kihara
Chapter 10 Artificial Intelligence-Driven Data Acquisition for Single-Particle Cryo-EM and Three-Dimensional Electron Diffraction
Koji Yonekura
Chapter 11 From Icy Specimens to Digital Insights: Integrating Sample Preparation, Data Analysis, and IT Infrastructure
Eric Shell, Karine Minari, and Vitor Hugo Balasco Serrão
SECTION III Cryo-EM in Virology and Enzymology: High-Resolution Structures of Chaperonins and Enzymes
Chapter 12 Cryo-EM Structures of Viral and Bacterial Chaperonins
Olga S. Sokolova and Tatiana B. Stanishneva-Konovalova
Chapter 13 Cryo-EM Structure of Monkeypox Virus DNA Polymerase Holoenzyme
Yi Shi and George F Gao
Chapter 14 Determining the High-Resolution Structure of the [NiFe]-hydrogenase Huc by Native Host Purification and Cryo-EM
Hari Venugopal, Ashleigh Kropp, Chris Greening, and Rhys Grinter
Chapter 15 Membrane Rupture and Lipid-Protein Interaction of ?-Hemolysin from Staphylococcus aureus Using Cryo-EM
Suman Mishra, Anupam Roy, Arnab Chatterjee, and Somnath Dutta
SECTION IV Cryo-EM in the Study of Membrane Biology: Probing the Structures of Membrane Proteins
Chapter 16 Historical Evolution and Perspective on Cryo-EM Analysis of Membrane Proteins
Youzhong Guo
Chapter 17 Single-Particle Cryogenic Electron Microscopy Unfolds Distinct Membrane Transporter
Folds: Focus on Autoinducer-2 Exporter Family
Radhika Khera
Chapter 18 Rhodopsin Dimers in the Retinal Rod Outer Segment
Sahil Gulati and Krzysztof Palczewski
Chapter 19 Cryo-EM Structures of the Fungal GPCR Dimer Ste2
Vaithish Velazhahan and Christopher G. Tate
Chapter 20 Human V-ATPases: Assembly, Function, and Regulation
Elizabeth Fosuah, Qingpeng Lin, and Tian-Min Fu
Chapter 21 Structure and Function of the Intracellular Calcium Channel Ryanodine Receptor
Marco C. Miotto
Chapter 22 Cryo-EM of Mitochondrial Membrane Proteins
Swati Yadav, Shaileshanand Jha, and Kutti R. Vinothkumar
SECTION V Cryo-EM in the Study of Molecular Biology: Structural Insights of Gene Machineries
Chapter 23 Imaging Structurally Dynamic Ribosomes with Cryogenic Electron Microscopy
Samantha M. Webster, Mira B. May, Barrett M. Powell, and Joseph H. Davis
Chapter 24 Cryo-EM in the Study of Ribosome Biogenesis
Ankita Arora and Prem S. Kaushal
Chapter 25 Cryo-EM Structures of Ribosomes from Human Pathogens: Implications for Drug Targeting
Ankit Dhur, Aneek Banerjee, Krishnamoorthi Srinivasan, and Jayati Sengupta
SECTION VI Cryo-EM in the Study of Neurobiology and Skin—Neuroreceptors to Ion Channels and Synaptic Transmission
Chapter 26 Cryo-EM Structural Features of Glutamate Receptors Involved in Synaptic Neurotransmission or Brain Pathology
Nebojša Bogdanovi?
Chapter 27 Cryo-EM Structures of Systemic Amyloid Fibrils
Binh An Nguyen, Maria del Carmen Fernandez-Ramirez, Shumaila Afrin, and Lorena Saelices
Chapter 28 Structural Insights into the 5-HT3A Receptor Revealed by Cryogenic Electron Microscopy (Cryo-EM)
Zhuowen Li, Nikhil Bharambe, and Sandip Basak
Chapter 29 Molecular Cryo-EM in the Study of Skin Tissue
Lars Norlen
SECTION VII Applications of Cryo-ET and Cryo-EM in the Biopharmaceutical Industry
Chapter 30 Emerging Technologies in Cryo-Electron Tomography
Kanika Khanna
Chapter 31 Cryo-Electron Tomographic Imaging of Membrane Proteins from Mitochondria and Chloroplasts
Tofayel Ahmed and Antara Saha
Chapter 32 Functional Studies of the Actin Cytoskeleton by Cryogenic Electron Tomography
Dorit Hanein and Niels Volkmann
Chapter 33 Cryo-EM in Drug Discovery
Youxin Kong, Maxime Killer, Madhumati Sevvana, and Chiara Rapisarda
Chapter 34 Applications of Single-Particle Analysis and Cryo-ET in Drug Discovery
Qing Yao
Index
