Methods in Molecular Biology

1. Defining a Genetic Ideotype for Crop Improvement Richard M. Trethowan 2. From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding Melissa Garcia and Diane E. Mather 3. Temperature Switch PCR (TSP) - A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms Thanh Le Phuoc and Kelvin Khoo 4. Multiplex-Ready Technology for Mid-Throughput Genotyping of Molecular Markers Julien Bonneau and Matthew Hayden 5. Genotyping by High-Resolution Melting Analysis Elise J. Tucker and Bao Lam Huynh 6. Bi-Allelic SNP Genotyping Using the TaqMan(R) Assay John Woodward 7. SNP Genotyping: The KASPTM Assay Chunlin He, John Holme, and Jeffrey Anthony 8. Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding S. Perez-Elizalde, J. J. Ceron-Rojas, J. Crossa, Delphine Fleury, and G. Alvarado 9. OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs Fabio Valente, Franck Gauthier, Nicolas Bardol, Guylaine Blanc, Johann Joets, Alain Charcosset, and Laurence Moreau 10. Genomic Selection in Plant Breeding M. A. Newell and J.-L Jannink 11. Simulated Breeding with QU-GENE Graphical User Interface Adrian Hathorn, Sctoo Chapman, and Mark Dieters 12. The Control of Recombination in Wheat by Ph1 and Its Use in Breeding Graham Moore 13. TILLING for Plant Breeding Peter Sharp and Chongmei Dong 14. In vitro Culture for Doubled Haploids: Tools for Molecular Breeding Sue Broughton, Parminder K. Sidhu, and Philip A. Davies 15. Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin Ainur Ismagul, Gulnur Oskakova, John C. Harris, and Serik Eliby 16. Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.) Ainur Ismagul, Iryna Mazonka, Corrine Callegari, and Serik Eliby 17. qPCR for Quantification of Transgene Expression and Determination of Transgene Copy Number Stephen J. Fletcher 18. High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics Nataliya Kovalchuk