Chromosomal Mutagenesis

Contents Preface Contributors Biallelic Gene Knockouts in Chinese Hamster Ovary Cells Naoko Yamane-Ohnuki, Kazuya Yamano, and Mitsuo Satoh Highly Proficient Gene Targeting by Homologous Recombination in the Human pre-B Cell Line Nalm-6 Noritaka Adachi, Aya Kurosawa, and Hideki Koyama Robust Cell Line Development Using Meganucleases Jean-Pierre Cabaniols and Frederic Paques Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells Matthew Porteus Gene Targeting in Drosophila and C. elegans with Zinc-finger Nucleases Dana Carroll, Kelly J. Beumer, J. Jason Morton, Ana Bozas and Jonathan K. Trautman Orpheus Recombination: A Comprehensive Bacteriophage System for Murine Targeting Vector Construction by Transplacement Knut Woltjen, Kenichi Ito, Teruhisa Tsuzuki, and Derrick E. Rancourt Transposon Mediated Mutagenesis in Somatic Cells: Identification of transposon-genomic DNA junctions David A. Largaespada and Lara S. Collier Insertional Mutagenesis of the Mouse Germline with Sleeping Beauty Transposition Junji Takeda, Zsuzsanna Izsvak, and Zoltan Ivics Conditional Gene Trapping Using the FLEx System Thomas Floss and Frank Schnutgen Steps Towards Targeted Insertional Mutagenesis with Class II Transposable Elements Sareina Chiung-Yuan Wu, Kommineni J. Maragathavally, Craig J. Coates and Joseph M. Kaminski Targeting integration of the Saccharomyces Ty5 retrotransposon Troy L. Brady, Clarice L. Schmidt and Daniel F. Voytas Site-Specific Chromosomal Integration Mediated by f C31 Integrase Annahita Keravala and Michele P. Calos Triplex-Mediated Gene Modification Erica B. Schleifman, Joanna Y. Chin, and Peter M. Glazer Allelic exchange of unmarked mutations in Mycobacterium tuberculosis. Martin S. Pavelka, Jr. Mycobacterial Recombineering Julia C. van Kessel and Graham F. Hatfull Chromosomal Engineering of Clostridium perfringens Using Group II Introns Phalguni Gupta and Yue Chen