Part I. Reviews: Cell Cycle Regulations 1. Cell Cycle-Regulated Transcription: Effectively Using a Genomics Toolbox Sara L. Bristow, Adam R. Leman, and Steven B. Haase 2. Cell Cycle Regulation by Checkpoints Kevin J. Barnum and Matthew O'Connell 3. Interplay Between the Cell Cycle and Double-Strand Break Response in Mammalian Cells Kate Beishline and Jane Azizkhan-Clifford 4. Cell Cycle Regulation by Protein Degradation Deana M. Koepp 5. Linking Chromosome Duplication to Segregation Via Sister Chromatid Cohesion Adam R. Leman and Eishi Noguchi 6. The Greatwall-PP2A Axis in Cell Cycle Control Peng Wang, Marcos Malumbres, and Vincent Archambault 7. Cell Cycle Regulation by the Nutrient-Sensing Mammalian Target of Rapamycin (mTOR) Pathway Elisabet Cuyas, Bruna Corominas-Faja, Jorge Joven, and Javier A. Menendez 8. The Senescence Arrest Program and the Cell Cycle Alessandro Bitto, Elizabeth Crowe, Chad Lerner, Claudio Torres, and Christian Sell 9. Oncogenic Ras Pushes (and Pulls) Cell Cycle Progression Through ERK Activation Paul M. Campbell 10. Cell Cycle Regulation During Viral Infection Sumedha Bagga and Michael J. Bouchard 11. The Roles of Cohesins in Mitosis, Meiosis, and Human Health and Disease Amanda S. Brooker and Karen M. Berkowitz 12. Introductory Review of Computational Cell Cycle Modeling Andres Kriete, Eishi Noguchi, and Christian Sell Part II. Protocols: Analyzing Cell Cycle Regulations 13. Cell Cycle Synchronization and Flow Cytometry Analysis of Mammalian Cells Naoko Yoshizawa-Sugata and Hisao Masai 14. Analyzing Transcription Dynamics During the Budding Yeast Cell Cycle Adam R. Leman, Sara L. Bristow, and Steven B. Haase 15. Analyzing Cell Cycle Checkpoints in Response to Ionizing Radiation in Mammalian Cells Bin Wang 16. Analyzing DNA Replication Checkpoint in Budding Yeast Nicole Hustedt and Kenji Shimada 17. Analyzing Cell Cycle-Dependent Degradation and Ubiquitination in Budding Yeast Dong-Hwan Kim and Deanna M. Koepp 18. Imaging Analysis of Cell Cycle-Dependent Degradation of Cdt1 in Mammalian Cells Yasushi Shiomi, Naohiro Suenaga, Miyuki Tanaka, Akiyo Hayashi, and Hideo Nishitani 19. PCNA-Dependent Ubiquitination of Cdt1 and p21 in Mammalian Cells Akiyo Hayashi, Naohiro Suenaga, Yasushi Shiomi, and Hideo Nishitani 20. Analyzing Cdc2/Cdk1 Activation During Stress Response in Schizosaccharomyces pombe Miguel A. Rodriguez-Gabriel 21. Analyzing Ras-Associated Cell Proliferation Signaling Matthew C. Stout, Edgar Asiimwe, James R. Birkenstamm, Su Yeon Kim and Paul M. Campbell 22. Telomere Regulation During Cell Cycle in Fission Yeast Bettina A. Moser, Ya-Ting Chang, and Toru M. Nakamura 23. Detecting Senescence: Methods and Approaches Elizabeth P. Crowe, Timothy Nacarelli, Alessandro Bitto, Chad Lerner, Christian Sell, and Claudio Torres Part III. Protocols: Analyzing Cell Cycle Events and Molecules 24. Analyzing RB and E2F During the G1/S Transition Michael J. Thwaites, Matthew J. Cecchini, and Frederick A. Dick 25. Analyzing G1/S Transcriptional Controls Steffi Klier, Sarah Farmer, and Robertus A. M. de Bruin 26. Analysis of Replication Timing Using Synchronized Budding Yeast Cultures Jie Peng, M. K. Raghuraman, and Wenyi Feng 27. Analysis of ssDNA Gaps and DSBs in Genetically Unstable Yeast Cultures Jie Peng, M. K. Raghuraman, and Wenyi Feng 28. Chromatin Fractionation Analysis of Licensing Factors in Mammalian Cells Hideo Nishitani, Masayuki Morino, Yusuke Murakami, Takeshi Maeda, and Yasushi Shiomi 29. Imaging Analysis to Determine Chromatin Binding of Licensing Factors MCM2-7 in Mammalian Cells Mayayuki Morino, Miyuki Tanaka, Yasuhi Shiomi, and Hideo Nishitani 30. Chromatin Immunoprecipitation to Investigate Origin Association of Replication Factors in Mammalian Cells Adam R. Leman and Eishi Noguchi 31. Live Cell Fluorescence Imaging for Phenotypic Analysis of Mitosis Sushama Sivakumar, John R Daum, and Gary J. Gorbsky 32. Analyzing Sister Chromatid Cohesion in Mammalian Cells Katherine M. Feeney, Laura McFarlane-Majeed, and Joanna L. Parish 33. Affinity Purification of Protein Complexes from Drosophila Embryos in Cell Cycle Studies Zoltan Lipinszki, Peng Wang, Rhys Grant, Catherine Lindon, Nikola S. Dzhindzhev, Pier Paolo D'Avino, Marcin R. Przewloka, David M. Glover, and Vincent Archambault 34. Tracking Histone Variant Nucleosomes Across the Human Cell Cycle Using Biophysical, Biochemical and Cytological Analyses Marcin P. Walkiewicz, Minh Bui, Delphine Quenet, and Yamini Dalal