Preface Contributors Part I: Model organisms 1. Use of Whole Embryo Culture for Studying Heart Development Calvin T. Hang, and Ching-Pin Chang 2. Quantifying Cardiac Functions in Embryonic and Adult Zebrafish Tiffany Hoage, Yonghe Ding, and Xiaolei Xu 3. Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting Ching-Pin Chang 4. Morpholino Injection in Xenopus Panna Tandon, Chris Showell, Kathleen Christine, and Frank L Conlon 5. Chicken Chorioallantoic Membrane Angiogenesis Model Domenico Ribatti 6. Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin 7. Whole-mount Confocal Microscopy for Vascular Branching Morphogenesis Yoh-suke Mukouyama, Jennifer James, Joseph Nam, and Yutaka Uchida 8. Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining Yang Liu, Marc Antoniyak, and Xu Peng 9. Miniaturized Assays of Angiogenesis in Vitro May J. Reed and Robert B. Vernon Part II: Cell and Molecular Biology Methods 10. Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay Ching-Pin Chang, Yiqin Xiong, and Bin Zhou. 11. Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor 12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining Yanping Zhang and L. Bruno Ruest 13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte Jieli Li, Marc Antonyak, and Xu Peng 14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng 15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells Bin Zhou and William T. Pu 16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization Jan Patterson, Rick Metz, and Emily Wilson 17. C-kit Expression Identifies Cardiac Precursor Cells Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny 18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols Dongfei Qi and Mingui Fu 19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja 20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales, Xu Peng, and David E. Dostal Part III: New Techniques 21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways Shenyuan Zhang and Hongying Zheng 22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor 12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining Yanping Zhang and L. Bruno Ruest 13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte Jieli Li, Marc Antonyak, and Xu Peng 14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng 15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells Bin Zhou and William T. Pu 16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization Jan Patterson, Rick Metz, and Emily Wilson 17. C-kit Expression Identifies Cardiac Precursor Cells Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny 18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols Dongfei Qi and Mingui Fu 19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja 20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales, Xu Peng, and David E. Dostal Part III: New Techniques 21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways Shenyuan Zhang and Hongying Zheng 22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Bin Zhou and William T. Pu 16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization Jan Patterson, Rick Metz, and Emily Wilson 17. C-kit Expression Identifies Cardiac Precursor Cells Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny 18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols Dongfei Qi and Mingui Fu 19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja 20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales, Xu Peng, and David E. Dostal Part III: New Techniques 21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways Shenyuan Zhang and Hongying Zheng 22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Michael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan David G. Taylor and Anupama Natarajan
