Methods in Molecular Biology

PART I. Introduction 1 Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior David W. Murhammer PART II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses 2 Introduction to Baculovirus Molecular Biology Barbara J. Kelly, Linda A. King And Robert D. Possee 3 Baculovirus Transfer Vectors Robert D. Possee And Linda A. King 4 Recombinant Baculovirus Isolation Linda A. King, Richard Hitchman And Robert D. Possee 5 Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System James A. Fornwald, Quinn Lu, Frederick M. Boyce And Robert S. Ames PART III. INSECT CELL CULTURE 6 Available Lepidopteran Insect Cell Lines Dwight E. Lynn and Robert E. Harrison 7 Lepidopteran Insect Cell Line Isolation from Insect Tissue Dwight E. Lynn 8 Development Of Serum-Free Media For Lepidopteran Insect Cell Lines Leslie C. L. Chan and Steven Reid 9 Routine Maintenance And Storage Of Lepidopteran Insect Cell Lines And Baculoviruses Dwight E. Lynn and Robert L. Harrison PART IV. Protein Production with Recombinant Baculoviruses 10 Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System Jian-Ping Yang 11 Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System Christine M. Thompson, Johnny Montes, Marc G. Aucoin and Amine A. Kamen 12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors Sue H. Kadwell and Laurie K. Overton 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae Elena Kovaleva and David C. Davis 14 Production Of Virus-Like Particles For Vaccination Christine M. Thompson, Marc G. Aucoin and Amine A. Kamen 15 Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells Stephanie Chen PART V. Recombinant Protein Production with Transformed Insect Cells 16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression Robert L. Harrison and Donald L. Jarvis 17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression Marija Backovic and Thomas Krey 18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression Robert L. Harrison and Donald L. Jarvis PART VI Baculovirus Development and Production for Use as Insecticides 19Introduction To The Use Of Baculoviruses As Biological Insecticides Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning 20 Baculovirus Insecticide Production In Insect Larvae Nikolai Van Beek and David C. Davis 21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System 22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants Hsuan-Chen Wu, Hyung Joon Cha and William E. Bentley 23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production Hsuan-Chen Wu, Yu-Chen Hu and William E. Bentley 24Gene Silencing In Insect Cells Using Rnai Hsuan-Chen Wu, John C. March and William E. Bentley 25 Using the Baculovirus/Insect Cell System to Study Apoptosis Nor Chejanovsky 26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells Christian Hofmann