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| Editors Preface | p. v |
| In Memory | p. 1 |
| Table of Contents | p. 3 |
| Starters | p. 11 |
| New Aspects of Fungal Starter Cultures for Fermented Foods | p. 13 |
| Abstract | p. 13 |
| Introduction | p. 13 |
| Penicillium nalgiovense | p. 15 |
| Taxonomic relationships at the molecular level | p. 15 |
| Penicillin production is a common feature of p.nalgiovense | p. 17 |
| Heterologous Gene Expression in P. nalgiovense | p. 20 |
| Heterologous Gene Expression in P. nalgiovense 2.4 Cloning of genes from P. Nalgiovense important for the fermentation process | p. 21 |
| Penicillium camemberti | p. 23 |
| Penicillium roqueforti | p. 25 |
| Conclusions | p. 27 |
| References | p. 27 |
| Starters for the Wine Industry | p. 31 |
| Abstract | p. 31 |
| Introduction | p. 31 |
| Yeast starters in winemaking | p. 32 |
| The objectives of yeast starters | p. 32 |
| Properties of yeast used as selective criteria for active dry yeast producers and winemakers | p. 34 |
| Evaluation of the settlement of active dry yeast during alcoholic fermentation | p. 37 |
| Malolactic starters in winemaking | p. 38 |
| Indications for use of malolactic starter and description | p. 39 |
| The influence of lactic acid bacteria starters on wine quality and their selection | p. 41 |
| Efficiency of malolactic starters | p. 42 |
| The future of starters for winemaking | p. 43 |
| Conclusion | p. 45 |
| References | p. 45 |
| Physiology, Biosynthesis and Metabolic Engineering | p. 49 |
| Metabolism and Lysine Biosynthesis Control in Brevibacterium Flavum: Impact of Stringent Response in Bacterial Cells | p. 51 |
| Abstract | p. 51 |
| Introduction | p. 51 |
| Materials and Methods | p. 52 |
| Results and Discussion | p. 52 |
| Conclusions | p. 56 |
| References | p. 57 |
| Molecular Breeding of Arming Yeasts with Hydrolytic Enzymes by Cell Surface Engineering | p. 59 |
| Abstract | p. 59 |
| Introduction | p. 60 |
| Principle of Cell Surface Engineering of Yeast | p. 63 |
| Display of Amylolytic Enzymes on the Yeast Cell Surface | p. 65 |
| Display of Cellulolytic Enzymes on the Yeast Cell Surface | p. 67 |
| Display of Lipase on the Yeast Cell Surface | p. 70 |
| Cell Surface Engineering as a Novel Field of Biotechnology | p. 70 |
| References | p. 71 |
| Metabolic Pathway Analysis of Saccharomyces Cerevisiae | p. 75 |
| Abstract | p. 75 |
| Introduction | p. 75 |
| Metabolic pathway analysis | p. 76 |
| Metabolic control analysis | p. 76 |
| Metabolic flux analysis | p. 77 |
| Steady-state continuous cultivation - an excellent tool for metabolic pathway analysis | p. 79 |
| Metabolic pathway analysis applied to Saccharomyces cerevisiae | p. 80 |
| Kinetic studies of the glycolysis | p. 80 |
| Metabolic pathway analysis of the galactose metabolism | p. 81 |
| Acknowledgements | p. 85 |
| References | p. 85 |
| State Parameters and Culture Conditions | p. 87 |
| Effect of Aeration in Propagation on Surface Properties of Brewers' Yeast | p. 89 |
| Abstract | p. 89 |
| Introduction | p. 89 |
| Materials and Methods | p. 90 |
| Propagation conditions | p. 90 |
| Hydrophobicity | p. 90 |
| Surface charge | p. 91 |
| Flocculation | p. 92 |
| Results | p. 92 |
| Yield coefficients | p. 92 |
| Cell growth rates | p. 92 |
| Hydrophobicity | p. 93 |
| Zeta potential | p. 94 |
| Flocculation | p. 95 |
| Discussion | p. 96 |
| Conclusions | p. 98 |
| Acknowledgements | p. 98 |
| References | p. 99 |
| Effect of the Main Culture Parameters on the Growth and Production Coupling of Lactic Acid Bacteria | p. 101 |
| Abstract | p. 101 |
| Introduction | p. 101 |
| Materials and methods | p. 102 |
| Microorganism | p. 102 |
| Media | p. 102 |
| Fermentors and culture conditions | p. 102 |
| Analytical methods | p. 103 |
| Results and Discussion | p. 103 |
| Preculture conditions | p. 103 |
| Nutritional limitations | p. 105 |
| Initial lactate additions | p. 106 |
| Conclusions | p. 107 |
| Acknowledgements | p. 107 |
| References | p. 107 |
| Pseudohyphal and Invasive Growth in Saccharomyces Cerevisiae | p. 109 |
| Abstract | p. 109 |
| Introduction | p. 109 |
| Signal transduction in Saccharomyces cerevisiae | p. 110 |
| Molecular nature of signal transduction processes resulting in pseudohyphal differentiation | p. 112 |
| Signal transduction modules | p. 113 |
| Nutrient availability is sensed by permeases | p. 113 |
| Transmission via receptor associated elements | p. 114 |
| Intermediate signal transduction modules | p. 116 |
| Transcriptional regulators | p. 122 |
| Ste12p and Tec1 | p. 123 |
| Msn1p and Mss11p: Central elements in the pseudohyphal growth pathway | p. 123 |
| Sfl1p, Sok2p and Flo8p: Factors depending on the cAMP dependent kinase | p. 124 |
| Other factors | p. 125 |
| Effector proteins | p. 125 |
| MUC1, a gene encoding a mucin-like protein subjected to complex transcriptional regulation | p. 126 |
| Starch degrading enzymes: a direct metabolic link | p. 127 |
| Scientific and industrial relevance | p. 127 |
| Acknowledgements | p. 129 |
| References | p. 129 |
| Microbial Production of the Biodegradable Polyester Poly-3-Hydroxybutyrate (PHB) from Azotobacter Chroococcum 6B: Relation between PHB Molecular Weight, Thermal Stability and Tensile Strength | p. 135 |
| Abstract | p. 135 |
| Materials and methods | p. 135 |
| Microorganism and culture media | p. 135 |
| Fermentor experiments | p. 135 |
| Extraction and purification procedure | p. 136 |
| Analytical methods | p. 136 |
| Results and discussion | p. 136 |
| Effect of M[subscript w] on PHB thermal stability | p. 136 |
| Effect of aeration rate on PHB M[subscript w] | p. 137 |
| PHB tensile strength ([sigma]) at different M[subscript w] | p. 138 |
| PHB as a matrix for microencapsulation | p. 138 |
| Conclusions | p. 139 |
| References | p. 139 |
| Novel Approaches to the Study of Microorganisms | p. 141 |
| Sharing of Nutritional Resources in Bacterial Communities Determined by Isotopic Ratio Mass Spectrometry of Biomarkers | p. 143 |
| Introduction | p. 143 |
| Taxon specific biomarkers | p. 144 |
| Polar lipids | p. 144 |
| Outer membrane proteins | p. 145 |
| Isotopic fractionation in microorganisms | p. 146 |
| Carbon sharing in a pollutant degrading bacterial community | p. 147 |
| Origin and characteristics of the microbial consortium | p. 147 |
| Incorporation of [U-[superscript 13]C]-metabolites in microbial biomasses | p. 148 |
| Substrate competition | p. 149 |
| Community physiology of the microbial consortium | p. 150 |
| Outlook | p. 152 |
| Acknowledgement | p. 152 |
| References | p. 152 |
| A Comparison of the Mechanical Properties of Different Bacterial Species | p. 155 |
| Abstract | p. 155 |
| Introduction | p. 155 |
| Relative resistance of different microorganisms to mechanical disruption | p. 155 |
| Cell wall structure | p. 156 |
| Bacterial biomechanics | p. 157 |
| Micromanipulation | p. 158 |
| Materials and methods | p. 158 |
| The micromanipulation system | p. 158 |
| Culture conditions | p. 159 |
| Results and discussion | p. 160 |
| Conclusions and future developments | p. 161 |
| References | p. 162 |
| Novel Applications | p. 163 |
| Kocuria Rosea as a New Feather Degrading Bacteria | p. 165 |
| Abstract | p. 165 |
| Introduction | p. 165 |
| Isolation, identification and adaptation of feather-degrading microorganisms | p. 166 |
| Isolation and degradation of feathers by a microbial isolate | p. 166 |
| Morphological and ultrastructural characteristics of the feather-degrading isolate | p. 168 |
| Microbial growth and feather degradation | p. 168 |
| Effect of quantity of feathers | p. 168 |
| Effect of culture temperature on feather degradation and growth of LPB-3 | p. 171 |
| Kinetic fermentation | p. 171 |
| Industrial applications | p. 171 |
| Fermented feather meal | p. 171 |
| Enzymes | p. 173 |
| Pigments | p. 173 |
| Acknowledgements | p. 174 |
| References | p. 174 |
| Comparison of Pb[superscript 2+] Removal Characteristics Between Biomaterials and Non-Biomaterials | p. 177 |
| Abstract | p. 177 |
| Introduction | p. 177 |
| Materials and methods | p. 178 |
| Materials | p. 178 |
| Microorganisms and culture conditions | p. 178 |
| Pb[superscript 2+] removal experiment | p. 178 |
| Results and discussion | p. 179 |
| Pb[superscript 2+] removal characteristics | p. 179 |
| Initial Pb[superscript 2+] removal rate | p. 182 |
| Conclusions | p. 183 |
| References | p. 183 |
| Hydrocarbon Utilisation by Streptomyces Soil Bacteria | p. 185 |
| Abstract | p. 185 |
| Materials and methods | p. 185 |
| Test organisms. oligocarbophylic streptomyces | p. 185 |
| Biomass preparation | p. 186 |
| Incorporation of radioactivity from labelled n-Hexadecane into mycelia | p. 186 |
| Fluorescence measurements | p. 186 |
| Analysis of fatty acids | p. 187 |
| Investigations with GTP analogues | p. 187 |
| Results and discussion | p. 187 |
| Conclusion | p. 190 |
| References | p. 190 |
| Food Security and Food Preservation | p. 191 |
| Molecular Detection and Typing of Foodborne Bacterial Pathogens: a Review | p. 193 |
| Abstract | p. 193 |
| Introduction | p. 194 |
| Characteristics of the foodborne bacterial pathogens | p. 194 |
| Molecular detection and identification of foodborne bacterial pathogens | p. 198 |
| Nucleic acid based identification methods | p. 198 |
| The use of virulence genes as target for molecular identification | p. 198 |
| The use of RRNA genes as target for molecular identification | p. 199 |
| The use of specific sequences with a known or unknown function as target for molecular identification | p. 200 |
| The available molecular identification systems | p. 201 |
| PCR detection of bacterial pathogens in food products | p. 203 |
| Influence of food components on PCR performance | p. 203 |
| Sensitivity and contamination of PCR | p. 203 |
| The detection of the viability of cells by DNA based technology | p. 204 |
| Evaluation and validation of DNA based methods | p. 205 |
| DNA amplification methods for quantification of foodborne pathogens | p. 207 |
| Molecular typing of foodborne bacterial pathogens | p. 208 |
| Terminology and general information | p. 208 |
| Necessity of bacterial typing of foodborne pathogens | p. 208 |
| Species-subspecies-variety-clone-strain-isolate | p. 209 |
| Molecular typing techniques used for bacterial pathogens | p. 210 |
| Analysis of DNA fingerprints | p. 219 |
| Prospects in molecular typing | p. 220 |
| Molecular typing of some specific bacterial foodborne pathogens | p. 221 |
| Salmonella | p. 221 |
| Campylobacter jejuni | p. 226 |
| Listeria monocytogenes | p. 227 |
| Escherichia coli 0157 | p. 228 |
| Some other foodborne bacterial pathogens | p. 229 |
| References | p. 229 |
| Bioencapsulation Technology in Meat Preservation | p. 239 |
| Abstract | p. 239 |
| Introduction | p. 240 |
| Meat preservation | p. 241 |
| Biological fermentation | p. 241 |
| Chemical acidification | p. 243 |
| The application of encapsulation technology to meat preservation | p. 243 |
| The application of encapsulation technology to a microbial fermentation | p. 243 |
| Encapsulation matrices and the encapsulation process | p. 244 |
| The benefits of meat starter culture encapsulation | p. 246 |
| Commercial applications | p. 247 |
| The application of encapsulation technology to chemical acidification | p. 248 |
| Encapsulation matrices and the encapsulation process | p. 248 |
| The benefits of acidulant encapsulation | p. 249 |
| Commercial availability | p. 249 |
| Control of emerging pathogens | p. 250 |
| The application of encapsulation technology to bacteriocin delivery | p. 251 |
| Bacteriocins | p. 251 |
| Nisin | p. 251 |
| Encapsulation of nisin | p. 252 |
| Conclusions and future work | p. 261 |
| References | p. 261 |
| Index | p. 267 |
| Table of Contents provided by Syndetics. All Rights Reserved. |
ISBN: 9780792368588
ISBN-10: 0792368584
Series: Focus on Biotechnology
Published: 31st May 2001
Format: Hardcover
Language: English
Number of Pages: 288
Audience: General Adult
Publisher: Springer Nature B.V.
Country of Publication: NL
Dimensions (cm): 24.77 x 16.51 x 1.91
Weight (kg): 0.58
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