Antibody Engineering

Part I. In silico Methods 1. Use of IMGT(R) Databases and Tools for Antibody Engineering and Humanization Marie-Paule Lefranc, Francois Ehrenmann, Chantal Ginestoux, Veronique Giudicelli, and Patrice Duroux 2. Computer-Assisted Modeling of Antibody Variable Domains Oscar H. P. Ramos Part II. Generation of Diversity 3. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells Lars Toleikis and Andre Frenzel 4. Human in Cell scFv Library from Infiltrating B Cell Sylvie Peraldi-Roux 5. Construction of Human Naive Antibody Gene Libraries Michael Hust, Andre Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dubel 6. Synthetic Customized scFv Libraries Gautier Robin and Pierre Martineau 7. Selection of Stable scFv Antibodies by Phage Display Eeva-Christine Brockmann 8. Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs Cecile Vincke, Carlos Gutierez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, and Serge Muyldermans 9. Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires Mischa Roland Muller, Ronan O'Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, and Caroline Jane Barelle 10. Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ Part IV. Selections of Lead Candidate 11. Phage Display and Selections on Purified Antigens Julie Matz and Patrick Chames 12. Phage Display and Selections on Cells Klervi Even-Desrumeaux and Patrick Chames 13. Humanization by CDR Grafting and Specificity-Determining Residue Grafting Jin Hong Kim and Hyo Jeong Hong 14. Humanization by Guided Selections Sang Jick Kim and Hyo Jeong Hong 15. Selection of Antibody Fragments by Yeast Display Nathalie Scholler 16. Evolution of Antibodies in vitro by Ribosome Display Bryan M. Edwards and Mingyue He 17. Mammalian Cell Display of Full Length IgG Chen Zhou and Wenyan (David) Shen Part V. Production of Recombinant Antibodies 18. Production of Antibody Fragments in Escherichia coli Tomohisa Katsuda, Hiroyuki Sonoda, and Hideki Yamaji 19. Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris Steve Schoonooghe, Jannick Leoen, and Jurgen Haustraete 20. Monoclonal Antibody Expression in Mammalian Cells Richard (Yi) Zhang and Wenyan (David) Shen 21. Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells Daniel X. Johansson, Thomas Krey, and Oskar Andersson 22. Production of Antibody Fragments Using the Baculovirus-Insect Cell System Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji 23. Transient and Stable Expression of Antibodies in Nicotiana Species J. Christopher Hall, Michael D. McLean and Freydoun Garabagi Part VI. Variable Domain Optimization 24. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance Stephen Hearty, Paul Leonard, and Richard O'Kennedy 25. Affinity Determination of Biotinylated Antibodies by Flow Cytometry Klervi Even-Desrumeaux and Patrick Chames 26. Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput Screening Laurence Renaut, Celine Monnet, Olivier Dubreuil, Ouafa Zaki, Fabien Crozet, Khalil Bouayadi, Hakim Kharrat and Philippe Mondon 27. Affinity Maturation by Semi Rational Approaches Rodrigo Barderas, Johan Desmet, Philippe Alard, and J. Ignacio Casal 28. Molecular Scanning Mutagenesis: Combining Ribosome Display, Random Mutagenesis and Bioinformatic Analysis for Protein Engineering Alfredo Darmanin-Sheehan, William James Jonathan Finlay Orla Cunningham, and Brian Joseph Fennell Part VII. Fc Engineering 29. Fucose-Targeted Glyco-Engineering of Pharmaceutical Cell Lines Christiane Ogorek, Ingo Jordan, Volker Sandig, and Hans Henning von Horsten 30. Fc engineering: Design, expression and functional characterization of antibody variants with improved effector function Stefanie Derer, Christian Kellner, Sven Berger, Thomas Valerius and Matthias Peipp 31. Fc engineering: Serum half life modulation through FcRn binding Tove Olafsen 32. Monoclonal Antibody Lead Characterization - In vitro and in vivo methods Axel Hernandez, Julie Parmentier, Youzhen Wang, Jane Cheng and Gadi Gazit Bornstein Part VIII. Innovative Formats 33. Production, generation and characterization of scFv TNF ligand fusion proteins Andrea Fick, Agnes Wyzgol and Harald Wajant 34. Antibody-IL-2 fusion proteins for tumor targeting Andreas A. Hombach and Hinrich Abken 35. Recombinant immunotoxins containing low endotoxins for clinical and animal studies Masanori Onda 36. Chimeric antigen receptors for T-cell based therapy Eleanor J. Cheadle, Vicky Sheard, Andreas A. Hombach, Markus Chmielewski, Tobias Riet, Cor Berrevoets, Erik Schooten, Cor Lamers, Hinrich Abken, Reno Debets, David E. Gilham 37. Selection and Use of Intracellular Antibodies (Intrabodies) Sandrine Moutel, Clement Nizak, and Franck Perez 38. Radiolabeled Antibodies for Imaging and Therapy Jacques Barbet, Manuel Bardies, Mickael Bourgeois, Jean-Francois Chatal, Michel Cherel, Francois Davodeau, Alain Faivre-Chauvet, Jean-Francois Gestin, and Francoise Kraeber-Bodere 39. Design and Production of Multimeric Antibody Fragments, Focused on Diabodies with Enhanced Clinical Efficacy Glenn A. Powers, Peter J. Hudson, and Michael P. Wheatcroft 40. Production of Bispecific Antibodies: Diabodies and Tandem scFv Nora Hornig and Aline Farber-Schwarz