Preface...Table of Contents...Contributing Authors...1. Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (a1b1y1)Julia A. Hubbard, Bing Xiao, and Jon R. Wilson2. Visualizing AMPK Drug Binding Sites through Crystallization of Full Length Phosphorylated a2b1y1 HeterotrimerChristopher G. Langendorf, Jonathan S. Oakhill, and Bruce E. Kemp3. Biophysical Interactions of Direct AMPK ActivatorsRavi G. Kurumbail, Graham M. West, Venkatasubramanian Dharmarajan, Kris A. Borzilleri, Jane M. Withka, Jessica Ward, Allan R. Reyes, Francis Rajamohan, Patrick R. Griffin, and Matthew F. Calabrese4. Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen RelationshipElite Possik and Arnim Pause5. Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPKFiona A. Fyffe, Simon A. Hawley, Alexander Gray, and D. Grahame Hardie6. Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK ss-Subunit Carbohydrate Binding ModulesPaul R. Gooley, Ann Koay, and Jesse I. Mobbs7. Bioinformatics Approach to Identify Novel AMPK TargetsBrendan Gongol, Traci Marin, David A. Johnson, and John Y-J. Shyy8. Studying AMPK in an Evolutionary ContextArpit Jain, Valentin Roustan, Wolfram Weckwerth, and Ingo Ebersberger9. AMPK Protein Interaction Analyses by Yeast Two-HybridPascual Sanz, Rosa Viana, and Maria Adelaida Garcia-Gimeno10. Transient Expression of AMPK Heterotrimer Complexes in Mammalian CellsJonathan S. Oakhill, John W. Scott, and Toby A. Dite11. Knockdown of Human AMPK using the CRISPR-Cas9 Genome-Editing SystemAdrien Grenier, Pierre Sujibert, Severine Olivier, Helene Guermouche, Johanna Mondesir, Olivier Kosmider, Benoit Viollet, and Jerome Tamburini12. Compound C/Dorsomorphin - Its Use and Misuse as an AMPK InhibitorBiplab Dasgupta and William Seibel13. Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by ImmunoprecipitationJesper B. Birk and Jorgen F. P. Wojtaszewski14. Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in Skeletal MuscleJesper B. Birk and Jorgen F. P. Wojtaszewski15. Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High Performance Liquid ChromatographyNoemi Garcia-Tardon and Bruno Guigas16. Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to ActivationSimon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans, and D. Grahame Hardie17. Cellular Application of Genetically-Encoded Sensors and Impeders of AMPKTakafumi Miyamoto, Elmer Rho, Allen Kim, and Takanari Inoue18. Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated OrganellesGuillaume Vial and Bruno Guigas19. Study of AMPK Regulated Metabolic Fluxes in Neurons using the Seahorse XFe AnalyzerClaudia Marinangeli, Jerome Kluza, Philippe Marchetti, Luc Buee, and Valerie Vingtdeux20. Investigating the Role of AMPK in InflammationSarah J. Mancini and Ian P. Salt21. Studying the Role of AMPK in Cardiac Hypertrophy and Protein SynthesisFlorence Mailleux, Christophe Beauloye, Jean-Luc Balligand, Sandrine Horman, and Luc Bertrand22. Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose UptakeJoost J.F.P. Luiken, Dietbert Neumann, Jan F.C. Glatz, Will A. Coumans, Dipanjan Chanda, and Miranda Nabben23. Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured CellsMarc Foretz and Benoit Viollet24. Studying the Role of AMPK in AutophagySarah Krieg, Bernhard Luscher, Jorg Vervoorts, and Marc Dohmen25. Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 AxisChen-Song Zhang, Mengqi Li, Yue Zong, and Sheng-Cai Lin26. Manipulation and Measurement of AMPK Activity in Pancreatic IsletsAida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc, and Guy A. Rutter27. Analyzing AMPK Function in the HypothalamusPatricia Seoane-Collazo and Miguel Lopez28. Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney ProteinsRenee Rao, Kazuhiro Omi, Roshan Rajani, Hui Li, and Nuria M. Pastor-Soler29. A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability upon Metabolic StressSebastien Denanglaire, Tiphene Pirnay, Oberdan Leo, and Fabienne Andris30. Methods to Evaluate AMPK Regulation of Macrophage Cholesterol HomeostasisNicholas D. LeBlond and Morgan D. Fullerton31. Modulation of Vascular Function by AMPK: Assessment of NO Bioavailability and Surrogates of Oxidative StressSwenja Kroller-Schon, Andreas Daiber, and Eberhard Schulz32. Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood VesselsQilong Wang and Ming-Hui Zou33. Studying the Role of AMPK in AngiogenesisKatrin Spengler, Silke Lindenmuller, Nderim Kryeziu, Anne Knierim, and Regine Heller34. Analysis of Muscle Stem Cell Fate through Modulation of AMPK ActivityMarine Theret, Linda Gsaier, Sabrina Ben Larbi, Michele Weiss-Gayet, and Remi Mounier35. Evaluating the Role of Host AMPK in Leishmania BurdenDiana Moreira, Jerome Estaquier, Anabela Cordeiro-da-Silva, and Ricardo Silvestre36. Analysis of Transgenerational Phenotypes Following Acute Atarvation in AMPK-Deficient C. elegansEmilie Demoinet and Richard Roy37. Human γ2 AMPK MutationsArash Yavari, Dhruv Sarma, and Eduardo B. Sternick
