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Affinity Chromatography : Practical and Theoretical Aspects - P. Mohr

Affinity Chromatography

Practical and Theoretical Aspects

By: P. Mohr

Hardcover Published: 22nd November 1985
ISBN: 9780824774684
Number Of Pages: 320

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Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.

Prefacep. iii
Introduction
Introductory Remarksp. 3
Referencesp. 5
History of Affinity Chromatographyp. 7
Referencesp. 13
General Problems
Solid Matrix Supportp. 19
General properties of the solid matrix supportp. 20
Biopolymersp. 21
Synthetic polymersp. 31
Inorganic supportsp. 34
Referencesp. 35
Chemical Modification of Supports and Immobilization of Affine Ligandsp. 41
Polysaccharidesp. 42
Polyacrylamidesp. 48
Porous glassp. 50
General reactions for coupling ligandsp. 50
Spacersp. 57
Blocking of unreacted groupsp. 59
Referencesp. 59
Choice of Affine Ligandp. 65
General aspectsp. 65
Coenzymesp. 67
Lectinsp. 75
Polynucleic acidsp. 78
Special variantsp. 79
Referencesp. 79
General Consideration of the Adsorption and Elution Stepp. 85
Influence of complex stabilityp. 85
Steric conditionsp. 89
Ligand concentrationp. 95
Nonspecific interactionp. 99
Physicochemical aspects of affinity chromatographyp. 100
Practical aspects of affinity chromatographyp. 108
Referencesp. 115
Biospecific Affinity Chromatography (Bioadsorption)
Enzymes and Effectorsp. 123
Possibilities and problemsp. 124
Affinity chromatography at subzero temperaturesp. 125
Referencesp. 128
Immunoaffinity Chromatography and Immunoassaysp. 131
Immunoaffinity chromatographyp. 131
Immunoassaysp. 135
Referencesp. 138
Nucleic Acidsp. 143
Poly(A)-affinity chromatographyp. 143
Poly(A)-agaroses as affinity supportsp. 145
Detection and isolation of mRNA by cDNA-cellulose affinity chromatographyp. 146
Nucleic acid dye chromatographyp. 146
Referencesp. 147
Lectins, Glycoproteins, Viruses, and Cellsp. 151
General considerationsp. 151
Separation of lectinsp. 152
Affinity chromatography of glycoproteins and glycoprotein conjugatesp. 154
Membrane-bound glycoconjugatesp. 154
Affinity chromatography of virusesp. 157
Affinity chromatography of cellsp. 160
Referencesp. 162
Integral Membrane Proteins--Amphiphatic Nature, Solubilization, and Peculiarities in Their Purification by Affinity Methodsp. 169
Classification of membrane proteinsp. 170
Molecular propertiesp. 171
Solubilizationp. 172
Affinity methodsp. 174
Referencesp. 179
Variants of Affinity Chromatography Other Than Bioadsorption
Dye-Ligand Chromatographyp. 189
Structure, properties, and immobilization of Cibacron Blue F3G-Ap. 190
Mechanism of dye-protein interactionp. 192
Purification of enzymesp. 195
Purification of blood proteinsp. 196
Other dyesp. 201
Referencesp. 204
Metal Chelate Affinity Chromatographyp. 209
Referencesp. 214
Charge Transfer Adsorption Chromatographyp. 215
General considerationsp. 215
Electron acceptor gelsp. 217
Electron donor gelsp. 220
Referencesp. 223
Hydrophobic Interaction Chromatographyp. 225
General considerationsp. 225
Effects of neutral saltsp. 229
Influence of electrostatic charges of the adsorbent materialp. 229
Protein separation on hydrophobicity gradientsp. 233
Hydrophobic-ionic chromatographyp. 234
Conclusionsp. 234
Referencesp. 238
Covalent Chromatography (Chemisorption)p. 243
Referencesp. 248
Related Techniques
Affinity Partitionp. 253
Referencesp. 256
Affinity Electrophoresisp. 259
Affinity electrophoresis in agarose gelsp. 259
Affinity electrophoresis in polyacrylamide gelsp. 263
Quantitative affinity electrophoresisp. 264
Correlation of affinity chromatography and affinity electrophoresisp. 269
Applicationsp. 269
Future developmentsp. 271
Referencesp. 276
High Performance Liquid Affinity Chromatographyp. 281
Referencesp. 284
Appendix
Concluding Remarksp. 287
Referencesp. 290
Indexp. 293
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9780824774684
ISBN-10: 082477468X
Series: Chromatographic Science Series : Book 33
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 320
Published: 22nd November 1985
Publisher: Taylor & Francis Inc
Country of Publication: US
Dimensions (cm): 27.9 x 21.6  x 2.03
Weight (kg): 0.58
Edition Number: 1

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