Methods in Molecular Biology

1. The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications Natalie S. Poulter, William T.E. Pitkeathly, Philip J. Smith, and Joshua Z. Rappoport 2. Two-Photon Excitation Microscopy And Its Applications In Neuroscience Ricardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, and Carlos Portera-Cailliau 3. Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy Francesco Pampaloni, Roli Richa, Nariman Ansari, and Ernst H.K. Stelzer 4. Fluorescence Microscopy Based RNA Interference Screening Manuel Gunkel, Nina Beil, Jurgen Beneke, Jurgen Reymann, and Holger Erfle 5. Fluorescence Resonance Energy Transfer Microscopy (FRET) Kasia M. Kedziora and Kees Jalink 6. Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy Yuansheng Sun, Vinod Jyothikumar, and Ammasi Periasamy 7. Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation Bart Geverts, Martin E. van Royen, and Adriaan B. Houtsmuller 8. Fluorescence Correlation Spectroscopy Mark A. Hink 9. Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules Suvrajit Saha, Riya Raghupathy, and Satyajit Mayor 10. Practical Structured-Illumination Microscopy E. Hesper Rego and Lin Shao 11. 4Pi Microscopy of the Nuclear Pore Complex Martin Kahms, Jana Huve, and Reiner Peters 12. Application Of STED Microscopy To Cell Biology Questions Natalia H. Revelo and Silvio O. Rizzoli 13. Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes Kelsey Temprine, Andrew G. York, and Hari Shroff 14. Direct Stochastic Optical Reconstruction Microscopy (dSTORM) Ulrike Endesfelder and Mike Heilemann 15. Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells Taofei Yin and Yi I. Wu