A Guide to Methods in the Biomedical Sciences

1: Detection And Analysis Of Proteins A. Introduction B. Basic methods for protein analysis C. Characterization of primary, secondary, tertiary and quaternary structures of proteins 2: Detection And Analysis Of Nucleic Acids A. Introduction B. Basic methods for nucleic acid analysis C. DNA sequencing D. Detection and quantitation of RNA 3: Recombinant DNA Techniques: Cloning And Manipulation Of DNA A. Introduction B. Plasmid and viral vectors C. Libraries D. Site Directed Mutagenesis E. Polymerase chain reaction (PCR) F. Screening for differentially expressed genes (DEGs) G. Promoter-protein interactions H. Silencing gene expression I. Forensics and DNA technology 4: Antibody-Based Techniques A. Introduction B. Monoclonal antibodies C. Purification and use of antibodies D. Flow cytometry and fluorescence activated cell sorting (FACS) E. Other assays using antibodies 5: Microscopy: Imaging Of Biological Specimens A. Introduction B. Light microscopy C. Fluorescence and laser confocal microarray microscopy D. Electron Microscopy (EM) E. Magnetic resonance imaging (MRI) 6: The Derivation And Manipulation Of Experimental Animals In Biomedical Sciences A. Introduction B. Inbred strains of mice C. Congenic strains of mice D. Transgenic mice E. Targeted transgenes: "knockout" and "knockin" mice F. Other uses of mice in biomedical research References Some excellent methodology manuals Index