Single-Channel Recording

Cover -- Contents -- Part I: INTRODUCTION TO PATCH CLAMPING -- Chapter 1 A Practical Guide to Patch Clamping -- 1. Introduction -- 2. Patch-Clamp Techniques -- 3. The Patch-Clamp Setup -- 4. Pipette Fabrication -- 5. Experimental Procedures -- 6. Caveats and Sources of Artifacts -- References -- Chapter 2 Tight-Seal Whole-Cell Recording -- 1. Introduction -- 2. Basic Procedures -- 3. The Whole-Cell Recording Configuration from an Electrical Point of View -- 4. The Whole-Cell Recording Configuration from a Chemical Point of View -- 5. Comparison of Whole-Cell Recording with Other Electrophysiological Methods to Record from Cells -- References -- Chapter 3 Guide to Data Acquisition and Analysis -- 1. Introduction -- 2. Data Acquisition and Preanalysis -- 3. Single-Channel Analysis -- 4. Analysis of Macroscopic Currents -- 5. Multipurpose Programs -- 6. Choices for Hardware and Software -- Referencesn -- Part II: METHODS -- Chapter 4 Electronic Design of the Patch Clamp -- 1. Introduction -- 2. Current-Measurement Circuitry -- 3. Background Noise in the Current-Voltage Converter -- 4. Capacitance Transient Cancellation -- 5. Series Resistance Compensation -- Chapter 5 Low-Noise Recording -- 1. Introduction -- 2. Types of Noise -- 3. A Strategy for Reducing Noise -- 4. Measured Noise -- 5. Perspectives -- 6. Noise-Dependent Resolution Limits of Channel-Open Time -- 7. Appendix -- Chapter 6 Voltage Offsets in Patch-Clamp Experiments -- 1. Introduction -- 2. Analysis of the Offset Problem -- 3. Three Strategies for Handling the Offset Problem -- 4. Conclusions -- References -- Chapter 7 Techniques for Membrane Capacitance Measurements -- 1. Introdution -- 2. Sequre-Wave Stimulation: The "Time Domine" Technique -- 3. Sinusoidal Excitation -- 4. Stimulation with Signals Containing Multispectral Components -- 5. Resolution Limits of Membrane Capacitance Measurements -- 6. Practical Application, Hints, and Warnings -- Appendix A. Phase-Sensitive Detection -- Appendix B. Derivation of Equation 38 -- References -- Chapter 8 Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices -- 1. Introduction -- 2. Preparation of Brain Slices -- 3. Visualization of Nerve Cells Using IR-DIC -- 4. The "Blow and Seal" Technique -- 5. Examples of Recordings from Dendrites -- 6. Discussion and Conclusions -- References -- Chapter 9 Patch Clamp and Calcium Imaging in Brain Slices -- 1. Introduction -- 2. Setup -- 3. Procedures and Techniques -- 4. Examples and Applications -- References -- Chapter 10 Fast Application of Agonists to Isolated Membrane Patches -- 1. Introduction and History -- 2. Application Pipettes -- 3. Piezoelectric Elements and Power Supplies -- 4. Tests of the Reliability of Solution Exchange -- 5. Examples -- References -- Chapter 11 Electrochemical Detection of Secretion from Single Cells -- 1. Introduction -- 2. Principle -- 3. Setup -- 4. Experimental Considerations and Analysis -- 5. Conclusions and Prospects -- Appendix -- References -- Chapter 12 Technical Approaches to Studying Specific Properties of Ion Channels in Plants -- 1. Introduction: Why Do Plants Need Ion Channels? -- 2. Experimental Procedures -- 3. Outlook -- References -- Chapter 13 The Gi.