Multi-modality Microscopy

By: Hanry Yu (Editor), Ping-chin Cheng (Editor), Pao-chun Lin (Editor), Fu-jen Kao (Editor)

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Hardcover | 10 May 2006

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316 Pages

Dimensions(cm)
22.86 x 15.88 x 1.91

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This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engineering.

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Preface	p. v
Basic Principles of Imaging	p. 1
Light Microscopy	p. 1
Image Acquisition	p. 8
Data Correction	p. 14
Video Enhanced Contrast	p. 19
Image Processing	p. 25
Deconvolution	p. 40
Three-Dimensional Rendering	p. 63
References	p. 70
Optics for the Biologist: What You Need to Know to Make Your Light Microscope Work Properly	p. 75
Introduction	p. 75
The Problem of Spheres	p. 76
'Tube' Length and Magnification	p. 80
What to Do?	p. 89
Intermediate Summary	p. 90
Detecting Spherical Aberration	p. 90
How Do You Correct Spherical Aberration?	p. 93
Upon Reflection	p. 100
Conclusion	p. 104
References	p. 104
Spherical Aberration in Confocal Microscopy	p. 105
Introduction	p. 105
Aberration Introduced by Refractive Index Mismatch	p. 110
Compensation by Alteration of Immersion Medium Refractive Index	p. 114
Validity of the Debye Approximation in the Presence of Spherical Aberration	p. 117
Conclusion	p. 122
Acknowledgements	p. 122
References	p. 123
Deconvolution and Image Quality Control - Valuable Tools in Multi-Dimensional Light Microscopy	p. 125
Introduction	p. 126
Background	p. 127
Image Restoration	p. 130
Image Quality Control	p. 138
Conclusion	p. 145
Acknowledgements	p. 146
References	p. 147
Quantitative Polarised Light Microscopy	p. 150
Introduction	p. 150
Design Outline	p. 151
The Experimental System	p. 154
Examples of Images	p. 155
Conclusion	p. 160
Acknowledgements	p. 161
References	p. 161
Multi-Photon Scanning Microscopy Using a Femtosecond Cr:forsterite Laser	p. 162
Introduction	p. 162
Materials and Methods	p. 164
Results and Discussion	p. 168
Summary	p. 175
Acknowledgements	p. 175
References	p. 176
Design and Performance Evaluation of a Multi-Functional Microscopy	p. 178
Introduction	p. 178
Theory	p. 179
Experimental Results	p. 185
Conclusions	p. 187
References	p. 187
Zeeman Laser Scanning Confocal Microscopy in Turbid Media	p. 188
Introduction	p. 188
Experimental Setup	p. 190
Conclusion	p. 195
Acknowledgements	p. 195
References	p. 196
A Method for Real-Time Confocal Imaging of Substrate Surfaces During Active Air Abrasion Cutting: The Cutting Edge of Air Abrasion	p. 197
Introduction	p. 197
Materials and Methods	p. 201
Results	p. 205
Discussion	p. 212
Summary	p. 215
Acknowledgements	p. 216
References	p. 216
Three-Dimensional Optical Data Storage with a Multi-layered Recording Medium	p. 219
Introduction	p. 219
Bit-Oriented 3-D Memory	p. 221
Multi-Structured Recording Medium for Reading with Reflection Confocal Microscope	p. 223
Two-Photon Recording in Multi-Structured Medium	p. 228
Discussion and Conclusion	p. 229
References	p. 230
The Studies of Single Luminescent Conjugated Polymers	p. 232
Introduction	p. 232
Experimental	p. 233
Results and Discussion	p. 234
Conclusion	p. 236
References	p. 237
Confocal Microscopy of GFP-Like Pigments in Corals	p. 238
Introduction	p. 238
Materials and Methods	p. 239
Results and Discussion	p. 240
Conclusion	p. 243
Acknowledgements	p. 244
References	p. 244
Three-Dimensional Localisation of Fluorescence Resonance Energy Transfer in Living Cells under Two-Photon Excitation	p. 246
Introduction	p. 246
Experimental Results	p. 248
Conclusion	p. 256
Acknowledgements	p. 256
References	p. 256
Multi-Dimensional Imaging of Cell- and Tissue-Engineered Constructs	p. 257
Introduction	p. 257
Natural Spheroids or Engineered Cell Aggregates	p. 259
Micro-capsules	p. 265
Porous Scaffold-based 3-D Tissue Constructs	p. 267
Micro-Fluidic Channels	p. 271
Three-Dimensional Tissue Slices	p. 274
Conclusion and Future Work	p. 279
References	p. 280
Two-Photon Excited Fluorescent Spectra of Common Biological Dyes	p. 289
Color Figures	p. 291
Index	p. 299
Table of Contents provided by Ingram. All Rights Reserved.

Multi-modality Microscopy

At a Glance

Hardcover

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How to return your order

Defective items

You Can Find This Book In

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