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Cell Biology : A Laboratory Handbook - Julio E Celis

Cell Biology

A Laboratory Handbook

By: Julio E Celis (Editor)

eText | 10 May 2014 | Edition Number 1

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Cell Biology: A Laboratory Handbook, Volume 3 is a handbook on cell biology and covers topics ranging from transfer of macromolecules and small molecules to cloning of embryos, transgenics, and gene targeting. Cell-free extracts, permeabilized cell systems, and expression systems are also discussed, along with proteins.

Comprised of 58 chapters, this volume begins with a detailed account of microinjection of RNA, DNA, and proteins into somatic cells, followed by an analysis of computer-automated capillary microinjection of macromolecules into living cells. The reader is then introduced to syringe loading as a method for inserting macromolecules into cells in suspension; electroporation of cells; and the use of liposomes in drug targeting. Subsequent chapters focus on the cloning of rabbit embryos by nuclear transplantation; gene targeting by homologous recombination in embryonic stem cells; production and isolation of recombinant viruses; and gel electrophoresis.

This book will be of interest to geneticists and molecular biologists.
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Non-FictionScienceBiology, Life SciencesBiochemistryCellular BiologyPhysicsApplied PhysicsBiophysics

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