Bioprocess Engineering and Technology

Upstream Process

By: Biswanath Bhunia

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eText | 26 November 2025 | Edition Number 1

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This book discusses the various cellular interventions that are required for the upstream processes in bioprocess engineering such as growth requirement of different cell types, their maintenance, and various recombinant strategies/tools available to identify and develop a cellular factory for high-level synthesis of a desired product. Bioprocess Engineering and Technology: Upstream Process also deals with the various types of reactors, process design and development (media, conditions, mode of operation, sterilisation, selectivity), reactor design and construction, scale-up, process analytical technologies, and process control. The book examines upstream processes which include the selection of cell factories and strain engineering approaches, enzyme kinetics, growth kinetics, fermentation, downstream processing, process design, reactor design, and scale-up. It also includes case studies that will assist readers to develop the upstream processes involved in an industrial-scale bioprocess.

This book is intended for students and researchers looking to develop the upstream processes involved in an industrial-scale bioprocessing operation.