
Practical HPLC Method Development
By: Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch
eBook | 3 December 2012 | Edition Number 2
At a Glance
800 Pages
eBook
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.
on
Basics of Separation.
Detection Sensitivity and Selectivity.
Sample Preparation.
The Column.
Non-Ionic Samples: Reversed- and Normal-Phase HPLC.
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.
Gradient Elution.
Systematic Approach to the Reversed-Phase Separation of Regular Samples.
Computer-Assisted Method Development.
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
Chiral Separations.
Preparative HPLC Separation.
Quantitation (Including Trace Analysis).
Completing the Method: Validation and Transfer.
Appendices.
Index.
ISBN: 9781118591512
ISBN-10: 1118591518
Published: 3rd December 2012
Format: ePUB
Language: English
Number of Pages: 800
Audience: Professional and Scholarly
Publisher: Wiley
Country of Publication: US
Edition Number: 2
























