This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.
Preface... Table of Contents... Contributors... Part I Wheat Rust Surveillance and Genotyping 1. Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping Sajid Ali and David Hodson 2. Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance Vanessa Bueno-Sancho, Daniel C.E. Bunting, Luis J. Yanes, Kentaro Yoshida, and Diane G.O. Saunders 3. Race Typing of Puccinia striiformis on Wheat Mogens S. Hovmoller, Julian Rodriguez-Algaba, Tine Thach, and Chris K. Sorensen 4. Assessment of Aggressiveness of Puccinina striiformis on Wheat Chris K. Sorensen, Tine Thach, and Mogens S. Hovmoller 5. Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing Benjamin Schwessinger and John P. Rathjen 6. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis Sajid Ali, Muhammad R. Khan, Angelique Gautier, Zahoor A. Swati, and Stephanie Walter Part II Molecular Pathogenicity 7. Computational Methods for Predicting Effectors in Rust Pathogens Jana Sperschneider, Peter N, Dodds, Jennifer M. Taylor, and Sebastien Duplessis 8. Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana Benjamin Petre, Joe Win, Frank L.H. Menke, and Sophien Kamoun 9. Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction Semra Hasancebi 10. Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing Vinay Panwar and Guus Bakkeren 11. Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi Veronica Roman-Reyna and John P. Rathjen Part III Genetics of Rust Resistance 12. Genetic Analysis of Resistance to Wheat Rusts Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, and Ravi P. Singh 13. Advances in Identification and Mapping of Rust Resistance Genes in Wheat Urmil Bansal and Harbans Bariana 14. Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, and W.J. Raupp 15. Application of Genomic Selection in Breeding Wheat for Rust Resistance Leonardo Ornella, Juan Manuel Gonzalez-Camacho, Susanne Dreisigacker, and Jose Crossa 16. Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions Adnan Riaz and Lee Hickey Part IV Rust Resistance Gene Isolation and Characterization 17. Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, and Evans Lagudah 18. Isolation of Wheat Genomic DNA for Gene Mapping and Cloning Guotai Yu, Asyraf Hatta, Sambasivam Periyannan, Evans Lagudah, and Brande B.H. Wulff 19. MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes Burkhard Steuernagel, Kamil Witek, Jonathan D.G. Jones, and Brande B.H. Wulff 20. Rapid Gene Isolation using MutChromSeq Burkhard Steuernagel, Jan Vrana, Miroslava Karafiatova, Brande B.H. Wulff, and Jaroslav Dolezel 21. Rapid Identification of Rust Resistance Genes through Cultivar-Specific de novo Chromosome Assemblies Anupriya K. Thind, Thomas Wicker, and Simon G. Krattinger 22. BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance Li Huang 23. Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene which Encodes a Hexose Transporter Ricky J. Milne, Katherine E. Dibley, and Evans S. Lagudah Part V Biocontrol Agents for Rust Management 24. Biocontrol Agents for Controlling Wheat Rust Siliang Huang and Fahu Pang
Series: Methods in Molecular Biology
Number Of Pages: 294
Published: 31st August 2017
Publisher: HUMANA PR
Country of Publication: US
Dimensions (cm): 25.4 x 17.81
Weight (kg): 7.25