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Vitamins and Coenzymes, Part J : Volume 280 - John N. Abelson

Vitamins and Coenzymes, Part J

Volume 280

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Donald B. McCormick (Editor), John W. Suttie (Editor), Conrad Wagner (Editor)

Hardcover Published: 1st January 1997
ISBN: 9780121821814
Number Of Pages: 496

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* This volume and its companion Volumes 279, 281, and 282 provide
* A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes
* Details on physical, chemical, and biological properties of vitamins and coenzymes
* Chemical and biological syntheses of vitamins, coenzymes, and their analogs
* Aspects of transport and metabolism of vitamins and coenzymes

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Folic Acid
Total Chemical Synthesis of Chirally Pure (6S)-Tetrahydrofolic Acid
Analysis of Folates Using Combined Affinity and Ion Pair Chromatography
Molar Quantitation of Folates by Gas Chromatograph-Mass Spectrometry
Microbiological Assay of Folates in 96-Well Microtiter Plates
Microbiological Assay for Serum, Plasma, and Red Cell Folate Using Cryopreserved Microtiter Plate Method
Determination of Tetrahydrobiopterin Biosynthetic Activities by High-Performance Liquid Chromatography with Fluorescence Detection
Microtiter Plate Assay for Biopterin Using Cryopreserved Crithidia fasciculata
Identification and Assay of Folate Catabolites in Human Urine
Enzymatic Determination of Folylpolyglutamate Pools
Synthesis and Interconversion of Reduced Folylpolyglumates
Chemical Synthesis of (6S)-5-Formyl-5,6,7,8-tetrahydropteroyl-poly-gamma-L-glutamates
Biotin Derivatives of Folate Compounds: Synthesis and Utilization for Visualization and Affinity Purification of Folate Transport Proteins
Preparation of Stable Isotopically Labeled Folates for in Vivo Investigation of Folate Absorption and Metabolism
Interconversion of 6- and 7-Substituted Tetrahydropterins via Enzyme-Generated 4a-Hydroxytetrahydroprotein Intermediates
Enzymatic Synthesis of 6R-U-14C Tetrahydrobiopterin from U-14CGTP
Use of 10-Formyldideazafolate as Substrate for Rat 10-Formyltetrahydrofolate
Human Cytosolic Folylpoly-gamma-Glutamate Synthase
Folylpolyglutamate Synthase from Higher Plants
Purification of Folate-Dependent Enzymes from Rabbit Liver
Human 5,10 Methenyltetrahydrofolate Synthetase
Mitochondrial NAD-Dependent Methylenetetrahydrofolate Dehydrogenase-Methenyltetrahydrofolate Cyclohydrolase
Monofunctional NAD-Dependent 5,10-Methylenetetrahydrofolate Dehydrogenase from Saccharomyces cerevisiae
Methionine Synthase from Pig Liver
Purification and Assay of Cobalamin-Dependent Methionine Synthase from Escherichia coli
Formyltetrahydrofolate Hydrolase from Escherichi coli
Use of 13C Nuclear Magnetic Resonance to Evaluate Metabolic Flux through Folate One-Carbon Pools in Saccharomyces cerevisiae
Vitamin B12 and Cobalamins
Use of Magnetic Field Effects to Study Coenzyme B12 Dependent Reactions
Analysis of Cobalamin and Cobalamin Analogs by Gas Chromatography-Mass Spectrometry
Expression of Functional Intrinsic Factor Using Recombinant Baculovirus
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821814
ISBN-10: 0121821811
Series: Methods in Enzymology : Book 280
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 496
Published: 1st January 1997
Country of Publication: US
Dimensions (cm): 23.29 x 15.65  x 2.69
Weight (kg): 0.85