The principal objective of this symposium was to bring together investigators from all over the world who work in diverse areas of research but share a common interest in the physiologic and pathophysiologic funetions of the nueleoside adenosine. In 1978, the "International Conference on Physiological and Regulatory Funetions of Adenosine and Adenine Nueleotides," held in Banff, Canada, served as an effective catalyst in enabling scientists with differ- ent points of view to exchange information and ideas on this subject. Since 1978, the amount of research on different as peets of adenosine metabolism, mechanism of action, transport, and function in various tissues (in both nor- maI and abnormal states) has increased exponentially, and there was an urgent need for another meeting for updating information on this burgeoning subject. The focus of the meeting was extremely sharp, but the scope of the subject material was very broad.
Scientists categorized as chemists, biochemists, physiologists, pharmacologists, and immunologists, working with cells and eelI fragments, with tissues such as heart, brain, musele, fat, kidney, and with human beings having immune deficiency diseases participated in the sym- posium. They had an opportunity to air their differences, reinforce their areas of agreement, and, most important, they educated each other with respeet to research on adenosine; it is hoped that they also enriched their own research programs from the information they gained from their fellow participants.
I. Adenosine Receptors.- 1. The complex structure and regulation of adenylate cyclase.- 2. Adenosine receptors and adenylate cyclase interactions.- 3. Adenosine receptors on vascular smooth muscle.- 4. A comparison of receptors for adenosine and adenine nucleotides.- 5. General aspects of ligand-binding phenomena.- 6. General aspects of binding of ligands to adenosine receptors.- 7. Binding of radioactive ligands to adenosine receptors in the central nervous system.- II. Adenosine Metabolism and Transport.- 8. Regulation of adenosine metabolism by 5?-nucleotidases.- 9. Metabolism of adenosine and sites of production in the heart.- 10. Interaction of adenosine with adenosine-binding protein, S-adenosylhomocysteine hydrolase.- 11. S-Adenosylhomocysteine hydrolase as a target in genetic and drug-induced deficiency of adenosine deaminase.- 12. Nucleoside transport in mammalian cells and interaction with intracellular metabolism.- 13. Inhibition of nucleoside transport.- III. Adenosine and Regulation of Immune Function.- 14. Mechanisms of toxicity of adenosine, 2-deoxyadenosine, and inhibitors of adenosine deaminase.- 15. Clinical expression of blocks of purine nucleotide degradation.- 16. Adenosine and cyclic nucleotides in modulation of immune responses.- 17. Mechanisms of adenosine and 2? -deoxyadenosine inhibition of immune function in mouse lymphocytes.- IV. Adenosine and Platelet Function.- 18. Receptors for nucleosides and nucleotides on blood platelets.- V. Vascular Effects of Adenosine.- 19. Blood flow regulation by adenosine in heart, brain, and skeletal muscle.- 20. Mechanisms of action of adenosine on vascular smooth muscle and cardiac cells.- 21. Adenosine uptake and adenine nucleotide metabolism by vascular endothelium.- 22. Adenine nucleotide and adenosine metabolism in cultured coronary endothelial cells: formation and release of adenine compounds and possible functional implications.- VI. Influence of Adenosine on Cardiac and Renal Function.- 23. Antiadrenergic effects of adenosine in the heart.- 24. Chronotropic and dromotropic effects of adenosine.- 25. Adenosine and renal function.- VII. Actions of Adenosine in the Nervous System.- 26. The role of adenosine in central neuromodulation.- 27. Synaptic modulation by adenosine: electro physiological and biochemical characteristics.- 28. Modulation of calcium currents by adenosine receptors on mammalian sympathetic neurons.- 29. Interactions of adenosine with other agents.- 30. Adenosine in the regulation of neurotransmitter release in the peripheral nervous system.- Abstracts.