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Target Assays for Modern Herbicides and Related Phytotoxic Compounds - Peter Boger

Target Assays for Modern Herbicides and Related Phytotoxic Compounds

Hardcover Published: 15th December 1992
ISBN: 9780873715393
Number Of Pages: 320

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Target Assays for Modern Herbicides and Related Phytotoxic Compounds is a laboratory guide that features 38 comprehensive contributions to determine and quantitate the inhibition activity of modern herbicides and related phytotoxic compounds at their targets. Using algal or higher-plant model species as well as cell-free enzymatic systems, assays are described for use with modern equipment typically found in a biochemical laboratory. Many details of the tests described in this volume are being published for the first time.

Assays discussed include carotenoid and chlorophyll biosynthesis and degradation, photosynthetic electron transport, amino acid biosynthesis, fatty acid formation, and cell division. Some model systems and related topics are also described. Each chapter represents an easy-to-read recipe with minimal theory including some key references for further reading. Original data from the experiments are provided, and most of the notable laboratories around the world are represented.

Target Assays for Modern Herbicides and Related Phytotoxic Compounds features updated methodology and procedures that will be a tremendous asset to plant biochemists; agriculture, plant protection, and weed control experts; agrochemical herbicide specialists in industry and government; and students in agricultural biochemistry and physiology.

Pigment Biosynthesis and Degradation
Spectral Determination of Carotenoid Precursors in Scenedesmus Cells Treated with Bleaching Herbicidesp. 3
Detection of Bleaching by Benzoyl Cyclohexanediones in Cress Seedlings by HPLC Separation and Determination of Carotenoidsp. 9
In Vitro Assay System for Phytoene Desaturase Inhibitors with Isolated Thylakoidsp. 15
Glutamate 1-Semialdehyde Aminotransferase as a Target for Herbicidesp. 21
Fluorometric Assay of Protoporphyrinogen Oxidase in Chloroplasts and in Plant, Yeast, and Mammalian Mitochondriap. 29
Inhibition of Protoporphyrinogen Oxidase from Maizep. 35
Short-Term Formation of Protoporphyrin-IX in Scenedesmus using Cyclic Imide Analoguesp. 43
Ethane Formation by Peroxidative Herbicidesp. 51
Peroxidizing Activity Determined by Cellular Leakagep. 61
Photosynthetic Electron Transport
A Simple Fluorometric Detection of Photosystem II Inhibitorsp. 69
In Vivo Measurements of Cytochrome f and the P515 Responsep. 75
Inhibition of the Photosynthetic Electron Transport Systemp. 83
Binding of Herbicides at the D1 Protein of the Photosynthetic Electron Transport Systemp. 93
Amino Acid Biosynthesis
Irreversible Inhibition of Glutamine Synthetase from Higher Plants by the Herbicide Phosphinothricinp. 103
The Buckwheat Assay for Glyphosate and Other Inhibitors of Aromatic Biosynthesisp. 109
EPSP Synthase: Isolation and Assayp. 115
Investigations on Herbicidal Acetolactate Synthase Inhibitors using Valine-Sensitive and Valine-Insensitive Escherichia coli Strainsp. 123
Assay of Acetolactate Synthase from Higher Plantsp. 131
Ketol-Acid Reductoisomerase from Plants: Extraction and Enzymatic Assayp. 137
Screening of Inhibitors (Antimetabolites) of the Biosynthesis or Function of Amino Acids or Vitamins with a Lemna Assayp. 143
Fatty Acid Formation
Assay of Plant Acetyl-CoA Carboxylase and Its Specific Inhibition by Some Graminicidesp. 153
Activity and Inhibition of Acetyl-CoA Carboxylase Determined by HPLCp. 161
A Novel Procedure for Measurement of Acetyl CoA Carboxylase in Comparison with the[superscript 14 CO subscript 2] Fixation Methodp. 167
Changes of Fatty Acid Profile Induced by Herbicidesp. 175
Inhibition of Cell Division
Components of Plant Growth and Its Measurementp. 185
Analysis of the Mitotic Index in Root Tip Meristems as a Tool to Determine Herbicide Cell Cycle Specificityp. 191
Estimates of Cell Cycle Duration in Meristems of Herbicide-Treated Plantsp. 197
Analysis of Cell Elongation as a Tool to Determine Herbicide Effects on Plant Growthp. 203
Immunofluorescence Microscopy of Tubulin to Determine Phytotoxin Effects on the Plant Cytoskeletonp. 211
Immunoblotting Protocol for the Characterization of Cytoskeletal Proteins Associated with Mitotic Disrupter Herbicidesp. 217
Model Species and Miscellaneous Topics
Inhibition of Plasma Membrane ATPasep. 225
Assays of Xenobiotics on Respiratory Activities in Isolated Plant Mitochondriap. 231
Rooting and Algal Tests for Herbicide Mode of Action Studiesp. 239
Growth Rate Determination of Lemna by Video Scan of the Leaf Surface Areap. 251
The Paraquat Bioassay to Evaluate Photosynthesis Inhibitionp. 257
The Starch Assay in Leaf Segmentsp. 263
Screening for Growth Retardants and Herbicides (Conductivity Measurement) with Heterotrophic Plant Cell Suspension Culturesp. 269
Screening of Peroxidizers that Cause an Accumulation of Photosensitive Tetrapyrroles (Qualitative Lemna Assay)p. 277
Affiliation Indexp. 285
General Indexp. 287
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780873715393
ISBN-10: 087371539X
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 320
Published: 15th December 1992
Country of Publication: US
Dimensions (cm): 24.77 x 16.51  x 2.54
Weight (kg): 0.64
Edition Number: 1

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