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Signalling by Inositides : A Practical Approach - Stephen Shears

Signalling by Inositides

A Practical Approach

By: Stephen Shears (Editor)

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Inositide research has infiltrated numerous branches of biochemistry, physiology, and molecular biology. Consequently, methodological inform ation has been scattered far and wide. This book unites a wide selecti on of the most fundamental and commonly used techniques. Contributors come from international signal transduction laboratories that have bee n actively involved in developing and refining these techniques. A uni que feature of the book is a catalog of non-commercial sources of synt hetic inositide analogues. Because there's an emphasis on broader appl ications instead of the more esoteric methods, this latest volume in t he Practical Approach series is useful as an introduction and a refres her for new and seasoned inositologists, respectively.

"The graphs, diagrams, tables, and few half-tone illustrations are of good to excellent quality...The book is small and easy to handle and read...Complete list of suppliers at the end of the book....This small, well-written book should be useful for those who do not regularly work in this field. It
contains clearly written and presented lab protocols that should be easy for anyone to follow and make work in their own laboratory."--Doody's Journal

1: Assaying inositol phospholipid turnover in plant cells 2: Measurement of inositol phosphate turnover in intact cells and cell-free systems 3: Mass assay of inositol and its use to assay inositol polyphosphates 4: Phospholipase C-gamma 1 5: Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-beta isoenzymes 6: Purification and assay of phospholipase C-delta 7: The purification and assay of inositol polyphosphate-binding proteins 8: Mass assay of inositol 1,4,5-trisphosphate and phosphatidylinositol 4,5-bisphosphate 9: Desalting inositol polyphosphates by dialysis 10: The Ins(1,4,5)P3 receptor 11: Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase 12: Phosphoinositide 3-kinase

ISBN: 9780199636389
ISBN-10: 0199636389
Series: Practical Approach Series
Audience: General
Format: Paperback
Language: English
Number Of Pages: 256
Published: 1st March 2002
Country of Publication: GB
Dimensions (cm): 23.39 x 15.6  x 1.35
Weight (kg): 0.36