Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology (TM) series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes.
Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.
From the reviews:
"Give us an update statement of the present day possible use of RNA to treat several diseases are well presented thanks to a 30 chapters walk in the RNA world. ... Clearly enough, the book is of great interest to the entire scientific community, no matter if basic researchers, teachers or clinicians!" (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)
1. RNA Modifications: A Mechanism that Modulates Gene Expression John Karijolich, Athena Kantartzis, and Yi-Tao Yu 2. Quantitative Analysis of RNA Modifications John Karijolich, Athena Kantartzis, and Yi-Tao Yu 3. Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference Mouldy Sioud 4. Progress in siRNA Delivery: A Multifunctional Approach by Polymeric Nanoparticles Weiwei Gao, Zeyu Xiao, Alex Radovic-Moreno, Jinjun Shi, Robert Langer, and Omid C. Farokhzad 5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells Anne Mobergslien and Mouldy Sioud 6. Systemic Delivery of Synthetic siRNAs Dag R. Sorensen and Mouldy Sioud 7. What Are the Key Targeted Delivery Technologies of siRNA Now? Mouldy Sioud 8. Using OligoWalk to Identify Efficient siRNA Sequences David H. Mathews 9. Jet-Injection of Short Hairpin RNA Encoding Vectors into Tumor Cells Wolfgang Walther, Ulrike Stein, and Hermann Lage 10. Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, and Debra Taxman 11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1 Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg 12. Functional Studies on RNA-Transfected Cell Microarrays Christina Saten Fjeldbo, Kristine Misund, Clara-Cecilie Gunther, Mette Langaas, Tonje Strommen Steigedal, Liv Thommesen, Astrid Lagreid, and Torunn Bruland 13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, and Jun Miyake 14. Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications Shao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin 15. Inhibition of the microRNA Pathway in Zebrafish by siRNA Anders Fjose and Xiao-Feng Zhao 16. Profiling of miRNA Expression and Prediction of Target Genes Mouldy Sioud 17. Small RNA Cloning Seungil Ro and Wei Yan 18. In situ Hybridization Detection of microRNAs Rui Song, Seungil Ro, and Wei Yan 19. Detection and Quantitative Analysis of Small RNAs by PCR Seungil Ro and Wei Yan 20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells Sang-Jin Lee, Seong-Wook Lee, Jin-Sook Jeong, and In-Hoo Kim 21. Design and Function of Triplex Hairpin Ribozymes Guillermo Aquino-Jarquin, Ramiro Rojas-Hernandez, and Luis Marat Alvarez-Salas 22. Catalytic M1GS RNA as an Antiviral Agent in Animals Yong Bai, Paul Jay Fannin Rider, and Fenyong Liu 23. Using Live Cells to Generate Aptamers for Cancer Study Ling Meng, Kwame Sefah, Dalia Lopez Colon, Hui Chen, Meghan O'Donoghue, Xiangling Xiong, and Weihong Tan 24. Primer-Free Aptamer Selection Using a Random DNA Library Weihua Pan and Gary A. Clawson 25. Development of TLR 7/8 Small RNA Antagonists Mouldy Sioud 26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-Triphosphate Mouldy Sioud 27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated through mRNA Electroporation An M.T. Van Nuffel, Jurgen Corthals, Bart Neyns, Carlo Heirman, Kris Thielemans, and Aude Bonehill 28. Non-MHC-Dependent Redirected T Cells Against Tumor Cells Hilde Almasbak, Marianne Lundby, and Anne-Marie Rasmussen 29. Overcoming Self-Tolerance to Tumor Antigens Mouldy Sioud 30. Recent Advances in Hematopoietic Stem Cell Transplantation and Perspectives of RNAi Applications Yngvar Floisand and Mouldy Sioud
Series: Methods in Molecular Biology : Book 629
Number Of Pages: 526
Published: 16th September 2010
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 25.4 x 17.78
Weight (kg): 1.15