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Restriction Endonucleases : Nucleic Acids and Molecular Biology - A. Pingoud

Restriction Endonucleases

Nucleic Acids and Molecular Biology

By: A. Pingoud (Editor)

Hardcover Published: 1st December 2004
ISBN: 9783540205029
Number Of Pages: 443

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Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

Survey and summary : a nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genesp. 1
Restriction-modification systems as minimal forms of lifep. 19
Molecular phylogenetics of restriction endonucleasesp. 63
Sliding or hopping? : how restriction enzymes find their way on DNAp. 95
The type I and III restriction endonucleases : structural elements in molecular motors that process DNAp. 111
The integration of recognition and cleavage : X-ray structures of pre-transition state complex, post-reactive complex and the DNA-free endonucleasep. 137
Structure and function of EcoRV endonucleasep. 179
Two of a kind : BamHI and BglIIp. 215
Structure and function of the tetrameric restriction enzymesp. 237
Structure and function of type IIE restriction endonucleases - or : from a plasmid that restricts phage replication to a new molecular DNA recognition mechanismp. 261
Analysis of type II restriction endonucleases that interact with two recognition sitesp. 297
The role of water in the EcoRI-DNA bindingp. 319
Role of metal ions in promoting DNA binding and cleavage by restriction endonucleasesp. 339
Restriction endonucleases : structure of the conserved catalytic core and the role of metal ions in DNA cleavagep. 361
Protein engineering of restriction enzymesp. 393
Engineering and applications of chimeric nucleasesp. 413
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9783540205029
ISBN-10: 3540205020
Series: Nucleic Acids and Molecular Biology
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 443
Published: 1st December 2004
Publisher: Springer-Verlag Berlin and Heidelberg Gmbh & Co. Kg
Country of Publication: DE
Dimensions (cm): 23.5 x 15.5  x 3.18
Weight (kg): 0.95