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Resin Microscopy and On-Section Immunocytochemistry : Structure and Bonding - Geoffrey R. Newman

Resin Microscopy and On-Section Immunocytochemistry

Structure and Bonding

Paperback Published: May 2009
ISBN: 9783540672777
Number Of Pages: 273

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Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.

From the reviews of the second edition:

"This comprehensive and valuable book is a must-have for the immunohistochemical laboratory. It extensively covers the field of onsection immunocytochemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling protocols a ] . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. a ] it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocytochemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)

Resin Embeddingp. 1
The Strategic Approachp. 3
The Resinsp. 34
Resin Embedding Protocols for Chemically Fixed Tissuep. 64
Cryotechniquesp. 88
Methods for Resin Polymerisationp. 109
Handling Resin Blocksp. 130
On-Section Immunolabellingp. 135
Strategies in Immunolabellingp. 137
General Considerationsp. 164
Immunolabelling Protocols for Resin Sectionsp. 195
Resin Embedding and Immunolabellingp. 215
Examples of Typical Resin Embedding Regimes for Immunocytochemistryp. 229
App. II: List of Suppliersp. 240
Referencesp. 246
Indexp. 265
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9783540672777
ISBN-10: 354067277X
Series: Structure and Bonding
Audience: General
Format: Paperback
Language: English
Number Of Pages: 273
Published: May 2009
Publisher: SPRINGER VERLAG GMBH
Country of Publication: DE
Dimensions (cm): 23.5 x 19.05  x 1.58
Weight (kg): 0.51
Edition Number: 2
Edition Type: Revised