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Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology : Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th-8th October 2000 - Otto-Wilhelm Merten

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th-8th October 2000

By: Otto-Wilhelm Merten (Editor), D. Mattanovich (Editor), C. Lang (Editor), Gerhard Larsson (Editor), P. Neubauer (Editor)

Hardcover Published: 30th November 2001
ISBN: 9780792371373
Number Of Pages: 400

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The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.

List of Editors
Foreword
Protein synthesis and co-translational folding in cell-free translation systemsp. 1
The cellular response to unfolded proteins in the endoplasmic reticulump. 17
The effects of recombinant protein expression on the growth and metabolism of mammalian cellsp. 29
Mapping stresses in Escherichia coli to improve yield. Examining global gene regulation and 'cell conditioning' strategiesp. 43
Cellular responses to strong overexpression of recombinant genes in Escherichia coli: DNA relaxation and cell death after induction of a-glucosidasep. 55
From Vitreoscilla hemoglobin (VHb) to a novel class of growth stimulating hemoglobin proteinsp. 75
Protein mass production in hybridomas and recombinant CHO cellsp. 89
Inducible gene copy number amplification for the production of heterologous proteins in Kluyveromyces lactisp. 99
Antibiotic-free plasmid selection and maintenance in Bacteriap. 111
Modelling of segregational plasmid instability of a recombinant strain suspension of Escherichia colip. 125
Production of optically pure aryl epoxides by recombinant E. coli carrying styrene monooxgenase: A new biocatalyst based on pseudomonas fluorescens ST genesp. 141
Translational problems associated with the rare arginine CGG in Escherichia coli. Framshifting at CGG codonsp. 151
Optimisation of the solubility of the recombinant Itk kinase domain in Escherichia colip. 159
Bacterial senescence and the oxidation paradoxp. 171
Metabolic approaches for the optimisation of recombinant fermentation processesp. 179
Control and optimisation of cellular bottlenecks in recombinant protein productionp. 189
Expression and fermentation strategies for recombinant protein production in Escherichia colip. 195
Overexpression of a Rhizopus oryzae lipase in Pichia pastoris strains containing multiple copies of the target genep. 259
Development of a heterologous gene expression system for use in Lactocossus lactis: A novel gram-positive expression systemp. 269
Metabolic network analysis for human therapeutic protein productions: effects of P/O ratiop. 277
Process-oriented metabolic engineering: cell lines with new properties in nutrient exploitation and protein glycosylationp. 289
Influence of the metabolic status of packaging cells on retroviral vector productionp. 303
Optimizing the production of recombinant prion protein from CHO cellsp. 319
Recombinant protein production by transient transfection of suspension-growing cellsp. 331
Production of recombinant human trypsinogen in Escherichia coli and Pichia pastoria: A comparison of expression systemsp. 339
Secretion of homologous and heterologous recombinant proteins in Escherichia coli and other gram-negative bacteria by using a new secretion systemp. 347
Monitoring of genes that respond to overproduction of insoluble recombinant proteins in Escherichia coli and Bacillus subtilisp. 359
Model supported optimization of fed-batch fermentations for recombinant protein productionp. 371
Protease secretion capacity and performance analysis of recombinant Bacillus speciesp. 383
Subject Indexp. 393
Authors Indexp. 399
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780792371373
ISBN-10: 0792371372
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 400
Published: 30th November 2001
Publisher: Springer
Country of Publication: NL
Dimensions (cm): 25.4 x 17.15  x 1.91
Weight (kg): 0.86