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Recombinant DNA, Part E : Volume 154 - John N. Abelson

Recombinant DNA, Part E

Volume 154

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Ray Wu (Editor), Lawrence Grossman (Editor)

Hardcover Published: 11th January 1988
ISBN: 9780121820558
Number Of Pages: 576

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Recombinant DNA methods are powerful, revolutionary techniques for at least two reasons.
First, they allow the isolation of single genes in large amounts from a pool of thousands or millions of genes. Second, the isolated genes or their regulatory regions can be modified at will and re-introduced into cells for expression at the RNA or protein levels. These attributes help to solve complex biological problems and to produce new and better products in the areas of health, agriculture and industry.
Both volume 154 and 155 include the descriptions of several useful new restriction enzymes and rapid methods for DNA sequence analysis, along with a number of other useful methods.

Industry Reviews

Praise for the Volume "This reviewer finds Methods in Enzymology Volume 154, Recombinant DNA Part E to be excellent reference book for persons working in molecular biology." --ADOLESCENT AND PEDIATRIC GYNECOLOGY Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Methods for Sequencing DNA
Methods for Sequencing of in Vitro Amplified DNA
Strategy for Producing Single-Stranded DNA in Polymerase Chain Reaction for Direct Genomic Sequencing
Sequencing Products of Polymerase Chain Reaction
One-Sided PCR for Amplification and Sequencing cDNA
Reverse Cloning Procedure for Rapid Generation of Subclones for DNA Sequencing
Simple Method for Generating Unidirectional Deletion Mutants for DNA Sequencing and Protein Engineering
Direct Sequencing of PCR Products from Low-Melting Temperature Agarose
Direct Sequencing of Polymerase Chain Reaction Products
Fluorescent and Radioactive Solid-Phase Dideoxy Sequencing of PCR Products in Microtiter Plates
Automated Fluorescent DNA Sequencing of PCR Products
Specific-Primer Directed DNA Sequence Analysis Using Automated Four-Color Fluorescence Detection and Labeled Primers
High Speed DNA Sequencing by Capillary Gel Electrophoresis
Microtiter Methods for Preparation and Fluorescent Sequencing of M13 Clones
DNA Sequencing with Direct Transfer Electrophoresis and Nonradioactive Detection
Adenine Specific Chemical DNA Sequencing Reaction
Direct DNA Sequencing of PCR Amplified Genomic DNA by Maxam-Gilbert Method
Direct Sequencing of
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121820558
ISBN-10: 0121820556
Series: Methods in Enzymology : Book 154
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 576
Published: 11th January 1988
Country of Publication: US
Dimensions (cm): 22.86 x 15.24  x 3.33
Weight (kg): 0.98

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