This is the last in a set of three books covering the practical aspects of receptor biochemistry, receptor-effector coupling, and neurotransmitter/hormone receptor-ligand interactions. It focuses on the preparation, performance and interpretation of receptor-ligand binding studies, which are widely used in mainstream pharmacology, both in academic laboratories and the pharmaceutical industry. Binding studies are difficult to effectively perform, hence expert guidance and many practical tips are given throughout. The book contains a wealth of valuable protocols, and will be a useful bench manual for researchers in pharmacology, neuroscience, molecular biology and biochemistry.
'This is an excellent multi-author book. The book represents very good value for the amount of information that it contains and will appeal to both the experienced and those starting receptor research. Selected chapters would be of relevance to undergraduates embarking on final year projects.'
Anthony P. Davenport and Peter Molenaar, Addenbrookes Hospital, Trends in Biochemical Sciences, Volume 17 No. 12, December 1992
'a very timely publication ... This book, together with Receptor Biochemistry and Receptor-Effector Coupling, the other two books in this series, would form an invaluable resource for receptor studies.'
A. Sivaprasadarao, Biomedical Education 21 (1) 1993
'a valuable bench manual for all researchers in biochemistry, molecular biology, neuroscience, and pharmacology who are interested in receptor function'
Anticancer REsearch 12: (1992)
Kevin G. McFarthing: Selection and synthesis of receptor-specific radioligands; A.D. Strosberg: Anti-receptor antibodies as ligands; J.O. Dolly: Neurotoxins as receptor ligands; E.C. Hulme & N.J.M. Birdsall: Strategy and tactics in receptor binding studies; E.C. Hulme & N.J. Buckley: Receptor preparations for binding studies; J-X. Wang, H.I. Yamamura, Wan Wang, & W.R. Roeske: The use of the filtration technique in In Vitro radioligand
binding assays for membrane-bound and solubilised receptors; E.C. Hulme: Centrifugation binding assays; P.G. Strange: Charcoal adsorption for separating bound and free ligand in radioligand binding assays; E.C. Hulme: Gel
filtration assays for solubilised receptors; H. Prinz: Receptor binding kinetics; J.W. Wells: Analysis and interpretation of binding data; Appendix 1: K.G. McFarthing: Radioligands for receptor binding: Amersham International; R.L.Young: Radiogands for receptor binding: Du Pont-New England Nuclear Corp.