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Proteome Analysis : Interpreting the Genome - Speicher

Proteome Analysis

Interpreting the Genome

By: Speicher

Hardcover

Published: 26th March 2004
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This book explores the current status of proteomics, an exciting new discipline, which is less than 10 years old. This new field has rapidly grown into a major commercial and research enterprise with great prospects for dramatically advancing our knowledge of basic biological and disease processes. The contributors to this book are an international panel of proteomics experts, who review and discuss the current status of specific technologies and approaches.
Proteomics represents an exciting new way to pursue biological and biomedical science at an unprecedented pace. Proteomics takes a broad, comprehensive, systematic approach to understanding biology that is generally unbiased and not dependent upon existing knowledge. The major components of proteomics from basic discovery using a range of alternative analytical methods to discovery validation and use for clinical applications are discussed. State-of-the-art protein profiling methods include high resolution two-dimensional gels, two-dimensional differential in-gel electrophoresis, LC-MS and LC-MS/MS using accurate mass tags, and protein identifications of proteins from gels using mass spectrometry methods are discussed in depth. Other chapters describe comprehensive characterization of proteomes using electrophoretic prefractionation and analyses of sub-proteomes based on specific posttranslational modifications including the phospho-proteome, the glyco-proteome, and nitrated proteins. These conventional proteome analysis chapters are complemented by discussion of emerging technologies and approaches such as affinity based biosensor proteomics as well as the use of protein microarrays, microfluidics and nanotechnology. Strategies for improving throughput by automation are also discussed. Additional chapters address the application of current proteome techniques to clinical problems and the availability of protein expression library resources for proteome studies.
- Authored by international experts in the field
- Covers a wide range of topics including 2-D gels, global proteomics using accurate mass tags, global proteomics using electrophoretic prefractionation, microfluidics, and nanotechnology
- Includes state-of-the-art protein profiling methods, and emerging technologies

Overview of proteome analysis
Protein profile comparisons of microorganisms, cells and tissues using 2-D gels
Protein profiling using 2-Dimensional Gel Electrophoresis (2D-DIGE) with multiple fluorescent tags
Electrophoretic prefractionation for comprehensive analysis of proteomes
Modification specific proteomics applied to protein glycosylation and nitration
Phosphoproteomics: Mass spectrometry based techniques for systematic phosphoprotein analysis
Protein identification by in-gel digestion and mass spectrometry
The use of accurate mass tags based upon high-throughput
Fourier transform ion cyclotron resonance mass spectrometry for global proteomic characterization
Clinical applications of proteomics
Affinity-based biosensors, microarrays and proteomics
Protein expression library resources for proteome studies
Automation of proteome analysis
Micro- and nanotechnology for proteomics
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780444510242
ISBN-10: 0444510249
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 394
Published: 26th March 2004
Publisher: Elsevier Science & Technology
Country of Publication: GB
Dimensions (cm): 25.4 x 17.78  x 2.54
Weight (kg): 0.86