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Protein Phosphorylation : Protein Phosphorylation, Part B : Part B Volume 201 - John N. Abelson

Protein Phosphorylation

Protein Phosphorylation, Part B : Part B Volume 201

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Tony Hunter (Editor), Bartholomew M. Sefton (Editor)

Hardcover

Published: 1st August 1991
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This volume provides a compilation of recent methods for studying protein phosphorylation.

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Analysis of Protein Phosphorylation: Phosphoamino Acid Determination
Separation of Phosphotyrosine, Phosphoserine, and Phosphothreonine by High-Performance Liquid Chromatography
Chemical Properties and Separation of Phosphoamino Acids by Thin-Layer Chromatography and/or Electrophoresis
Determination of Phosphoamino Acid Composition by Acid Hydrolysis of Protein Blotted to Immobilon
A Comparison of Three Methods for Detecting Tyrosine-Phosphorylated Proteins
Antibodies to Phosphoamino Acids: Immunoprecipitation and Immunoblotting
Generation and Use of Antibodies to Phosphothreonine
Generation and Use of Antiphosphotyrosine Antibodies Raised**against Bacterially Expressed abl Protein
Preparation and Use of Antiphosphotyrosine Antibodies to Study Structure and Function of Insulin Receptor
Generation of Monoclonal Antibodies against Phosphotyrosine and Their Use for Affinity Purification of Phosphotyrosine-Containing Proteins
Isolation of Tyrosine-Phosphorylated Proteins and Generation of Monoclonal Antibodies
Generation and Use of Antiphosphotyrosine Antibodies for Immunoblotting
Phosphopeptide Mapping and Phosphorylation Site Determination
Phosphopeptide Mapping and Phosphoamino Acid Analysis by Two-Dimensional Separation on Thin-Layer Cellulose Plates
Cyanogen Bromide Cleavage and Proteolytic Peptide Mapping of Proteins Immobilized to Membranes
In Vivo Phosphorylation States of Proteins: Fast Atom7079v, cont.**Bombardment Mass Spectrometry and Other Techniques
Determination and Location of Phosphoserine in Proteins and Peptides by Conversion to S-Ethylcysteine
Solid-Phase Sequencing of 32p.-Labeled Phosphopeptides at Picomole and Subpicomole Levels
Identification of Phosphorylation Sites in Peptides Using a Microsequencer
Sequence Analysis of Phosphotyrosine-Containing Peptides
Phosphopeptide Synthesis
Synthesis of O-Phosphoserine- and O-Phosphothreonine-Containing Peptides
Synthesis of O-Phosphotyrosine-Containing Peptides. Determination of Phosphorylation Stoichiometry
Measurement of Stoichiometry of Protein**Phosphorylation by Biosynthetic Labeling
Estimation of Phosphorylation Stoichiometry by Separation of Phosphorylated Isoforms
Microchemical Determination of Phosphate in Proteins Isolated from Polyacrylamide Gels
Production of Phosphorylation State-Specific Antibodies
Protein Kinase Inhibitors: Peptide Inhibitors
Pseudosubstrate-Based Peptide**Inhibitors
Inhibitor Protein of Adenosine Cyclic Monophosphate-Dependent Protein Kinase, and Peptides Derived from It, in Study of cAMP-Mediated Cellular Processes
Chemical Inhibitors
Use of Sphingosine as Inhibitor of Protein Kinase
Properties and Use of H-Series Compounds as Protein Kinase Inhibitors
Use and Specificity of Staurosporine, UCN-01, and Calphostin C as Protein Kinase Inhibitors
Inhibition of Protein Tyrosine Kinases by Tyrphostins
Use and Specificity of Genistein as Inhibitor of Protein-Tyrosine Kinases
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821029
ISBN-10: 0121821021
Series: Methods in Enzymology : Book 201
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 547
Published: 1st August 1991
Publisher: ACADEMIC PR INC
Country of Publication: US
Dimensions (cm): 22.86 x 15.24  x 3.18
Weight (kg): 0.94