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Protein Expression : A Practical Approach - S. J. Higgins

Protein Expression

A Practical Approach

By: S. J. Higgins (Editor), B. D. Hames (Editor)

Paperback

Published: 1st June 1999
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Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.

' The earlier volume on Transcription and Translation has been well used in my laboratory, and I am sure this update will become equally well thumbed' Microbiology Today

1: Protein expression in mammalian cells 2: Expression in Xenopus oocytes and cell-free extracts 3: Expressing cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris 4: Baculovirus expression systems 5: Protein synthesis in eukaryotic cell-free systems 6: Prokaryotic in vivo expression systems 7: Cell-free coupled transcription-translation systems from Escherischia Coli 8: Monitoring protein expression

ISBN: 9780199636235
ISBN-10: 0199636230
Series: Practical Approach Series
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 304
Published: 1st June 1999
Publisher: Oxford University Press
Country of Publication: GB
Dimensions (cm): 23.4 x 15.6  x 1.8
Weight (kg): 0.52