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Protein Engineering by Semisynthesis - Carmichael J. A. Wallace

Protein Engineering by Semisynthesis

Hardcover Published: 29th September 1999
ISBN: 9780849347276
Number Of Pages: 296

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As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis.
In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed.
By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide.
This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake.


Protein Semisynthesisp. 1
Methodological Advances in the Last Decadep. 25
Stereoselective and Chemoselective Religationp. 45
Insulin Semisynthesisp. 73
Ribonuclease Semisynthesisp. 103
Hemoglobin Semisynthesisp. 151
Protease Inhibitorsp. 209
Cytochrome c Semisynthesisp. 233
Indexp. 277
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780849347276
ISBN-10: 0849347270
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 296
Published: 29th September 1999
Publisher: Taylor & Francis Inc
Country of Publication: US
Dimensions (cm): 23.5 x 15.88  x 1.91
Weight (kg): 0.57
Edition Number: 1

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